Detailed information of TA system TA129420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2883657..2884288
• Replicon: chromosome
• Accession: NZ_CP041790
• Organism: Mycobacterium tuberculosis strain SEA17020024P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: FPK20_RS13375
• Protein ID: WP_003413174.1
• Coordinates: 2883911..2884288 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: FPK20_RS13370
• Protein ID: WP_003413167.1
• Coordinates: 2883657..2883914 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK20_RS13340	2879571..2879885	+	315	WP_009937839.1	hypothetical protein	-
FPK20_RS13345	2880181..2881392	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
FPK20_RS13350	2881519..2882178	+	660	WP_003900847.1	hypothetical protein	-
FPK20_RS13355	2882175..2882837	+	663	WP_003900848.1	hypothetical protein	-
FPK20_RS13360	2882834..2883112	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK20_RS13365	2883205..2883618	+	414	WP_003413164.1	PIN domain nuclease	-
FPK20_RS13370	2883657..2883914	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
FPK20_RS13375	2883911..2884288	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK20_RS13380	2884304..2884678	-	375	WP_003413177.1	hypothetical protein	-
FPK20_RS13385	2884778..2885173	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
FPK20_RS13390	2885170..2885415	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
FPK20_RS13395	2885826..2886245	-	420	WP_003413190.1	A24 family peptidase	-
FPK20_RS13400	2886257..2887066	-	810	WP_003413193.1	shikimate dehydrogenase	-
FPK20_RS13405	2887063..2888316	-	1254	WP_193018048.1	endolytic transglycosylase MltG	-
FPK20_RS13410	2888309..2888821	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T129420 WP_003413174.1 NZ_CP041790:2883911-2884288 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T129420 NZ_CP041790:2883911-2884288 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT129420 WP_003413167.1 NZ_CP041790:2883657-2883914 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT129420 NZ_CP041790:2883657-2883914 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5