Detailed information of TA system TA129397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244577..1245145
• Replicon: chromosome
• Accession: NZ_CP041790
• Organism: Mycobacterium tuberculosis strain SEA17020024P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FPK20_RS05865
• Protein ID: WP_193018119.1
• Coordinates: 1244771..1245145 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FPK20_RS05860
• Protein ID: WP_003405863.1
• Coordinates: 1244577..1244774 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK20_RS05840	1240618..1241256	-	639	WP_003898730.1	lipid droplet-associated protein	-
FPK20_RS05845	1241346..1242353	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FPK20_RS05850	1242370..1243353	-	984	WP_003903326.1	hypothetical protein	-
FPK20_RS05855	1243416..1244489	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FPK20_RS05860	1244577..1244774	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK20_RS05865	1244771..1245145	+	375	WP_193018119.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK20_RS05870	1245348..1246046	+	699	WP_003898733.1	hypothetical protein	-
FPK20_RS05875	1246164..1246349	+	186	WP_003901093.1	hypothetical protein	-
FPK20_RS05880	1246276..1246551	-	276	WP_003405867.1	hypothetical protein	-
FPK20_RS05885	1246794..1247117	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
FPK20_RS05890	1247132..1247842	-	711	WP_003911399.1	hypothetical protein	-
FPK20_RS05895	1247864..1248073	+	210	WP_003911400.1	hypothetical protein	-
FPK20_RS05900	1248025..1248732	-	708	Protein_1162	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13458.58 Da
• Isoelectric Point: 5.1881

>T129397 WP_193018119.1 NZ_CP041790:1244771-1245145 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLIIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T129397 NZ_CP041790:1244771-1245145 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCATCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT129397 WP_003405863.1 NZ_CP041790:1244577-1244774 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT129397 NZ_CP041790:1244577-1244774 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: G0TH07