Detailed information of TA system TA129351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2250771..2251397
• Replicon: chromosome
• Accession: NZ_CP041789
• Organism: Mycobacterium tuberculosis strain SEA17020028P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FPK21_RS10460
• Protein ID: WP_003410075.1
• Coordinates: 2250999..2251397 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FPK21_RS10455
• Protein ID: WP_003911750.1
• Coordinates: 2250771..2250998 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK21_RS10445	2248810..2249154	-	345	WP_003410065.1	ferredoxin family protein	-
FPK21_RS10450	2249343..2250512	-	1170	WP_003899126.1	ATP-binding protein	-
FPK21_RS10455	2250771..2250998	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FPK21_RS10460	2250999..2251397	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FPK21_RS10465	2251580..2252011	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FPK21_RS10470	2252112..2252546	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FPK21_RS10475	2252983..2253162	-	180	Protein_2070	hypothetical protein	-
FPK21_RS10480	2253223..2253870	+	648	WP_071854215.1	transposase	-
FPK21_RS10485	2253824..2254414	+	591	WP_003899131.1	IS110 family transposase	-
FPK21_RS10490	2254542..2255798	-	1257	WP_003902247.1	HNH endonuclease	-
FPK21_RS10495	2256152..2256352	+	201	Protein_2074	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T129351 WP_003410075.1 NZ_CP041789:2250999-2251397 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T129351 NZ_CP041789:2250999-2251397 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT129351 WP_003911750.1 NZ_CP041789:2250771-2250998 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT129351 NZ_CP041789:2250771-2250998 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8