Detailed information of TA system TA129269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 760002..760794
• Replicon: chromosome
• Accession: NZ_CP041788
• Organism: Mycobacterium tuberculosis strain SEA17020030P6C4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: FPK22_RS03485
• Protein ID: WP_003403386.1
• Coordinates: 760357..760794 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FPK22_RS03480
• Protein ID: WP_003403381.1
• Coordinates: 760002..760247 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK22_RS03450	755022..756527	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FPK22_RS03455	756608..757618	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FPK22_RS03460	758006..758389	-	384	WP_003403365.1	ribonuclease VapC6	-
FPK22_RS03465	758484..758639	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FPK22_RS03470	758715..759431	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FPK22_RS03475	759707..760015	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FPK22_RS03480	760002..760247	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FPK22_RS03485	760357..760794	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
FPK22_RS03490	760791..761045	-	255	WP_003911263.1	antitoxin VapB7	-
FPK22_RS03495	761159..763522	+	2364	WP_003898547.1	arylsulfatase AtsD	-
FPK22_RS03500	763585..763911	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPK22_RS03505	763823..764161	+	339	WP_003403405.1	PIN domain-containing protein	-
FPK22_RS03510	764158..764331	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T129269 WP_003403386.1 NZ_CP041788:c760794-760357 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T129269 NZ_CP041788:c760794-760357 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT129269 WP_003403381.1 NZ_CP041788:c760247-760002 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT129269 NZ_CP041788:c760247-760002 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0