Detailed information of TA system TA129206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3309973..3310610
• Replicon: chromosome
• Accession: NZ_CP041770
• Organism: Bacillus amyloliquefaciens strain DH8030

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FNS63_RS16245
• Protein ID: WP_003156187.1
• Coordinates: 3310260..3310610 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: FNS63_RS16240
• Protein ID: WP_003156188.1
• Coordinates: 3309973..3310254 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNS63_RS16220	3306338..3306937	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
FNS63_RS16225	3307030..3307395	+	366	WP_014304402.1	holo-ACP synthase	-
FNS63_RS16230	3307560..3308567	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
FNS63_RS16235	3308684..3309853	+	1170	WP_003156189.1	alanine racemase	-
FNS63_RS16240	3309973..3310254	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FNS63_RS16245	3310260..3310610	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FNS63_RS16250	3310728..3311549	+	822	WP_014304404.1	STAS domain-containing protein	-
FNS63_RS16255	3311554..3311919	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
FNS63_RS16260	3311922..3312323	+	402	WP_003156178.1	anti-sigma regulatory factor	-
FNS63_RS16265	3312335..3313342	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
FNS63_RS16270	3313406..3313735	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
FNS63_RS16275	3313732..3314214	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
FNS63_RS16280	3314180..3314968	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
FNS63_RS16285	3314968..3315570	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T129206 WP_003156187.1 NZ_CP041770:3310260-3310610 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T129206 NZ_CP041770:3310260-3310610 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT129206 WP_003156188.1 NZ_CP041770:3309973-3310254 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT129206 NZ_CP041770:3309973-3310254 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5