Detailed information of TA system TA129203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 525182..525835
• Replicon: chromosome
• Accession: NZ_CP041767
• Organism: Brevibacillus brevis strain B011

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: J2PLP3
• Locus tag: FO446_RS02850
• Protein ID: WP_005830205.1
• Coordinates: 525485..525835 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2GZH3
• Locus tag: FO446_RS02845
• Protein ID: WP_007721089.1
• Coordinates: 525182..525481 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO446_RS02830 (FO446_02865)	521747..523120	+	1374	WP_237899871.1	hypothetical protein	-
FO446_RS02835 (FO446_02870)	523305..523745	+	441	WP_047074011.1	hypothetical protein	-
FO446_RS02840 (FO446_02875)	523748..524935	+	1188	WP_237899872.1	alanine racemase	-
FO446_RS02845 (FO446_02880)	525182..525481	+	300	WP_007721089.1	CopG family ribbon-helix-helix protein	Antitoxin
FO446_RS02850 (FO446_02885)	525485..525835	+	351	WP_005830205.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FO446_RS02855 (FO446_02890)	526128..526457	+	330	WP_229088198.1	anti-sigma regulatory factor	-
FO446_RS02860 (FO446_02895)	526478..527479	+	1002	WP_173611931.1	PP2C family protein-serine/threonine phosphatase	-
FO446_RS02865 (FO446_02900)	527503..527829	+	327	WP_173611932.1	anti-sigma factor antagonist	-
FO446_RS02870 (FO446_02905)	527829..528323	+	495	WP_232774748.1	anti-sigma B factor RsbW	-
FO446_RS02875 (FO446_02910)	528292..529080	+	789	WP_088910503.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12917.86 Da
• Isoelectric Point: 4.8435

>T129203 WP_005830205.1 NZ_CP041767:525485-525835 [Brevibacillus brevis]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T129203 NZ_CP041767:525485-525835 [Brevibacillus brevis]
GTGATTGTCAAACGTGGCGATGTGTTTTTCGCGGACCTGTCCCCGGTCGTTGGCTCTGAGCAAGGAGGCGTACGGCCTGT
TTTAGTCATTCAAAACGACATTGGAAATCGATTTAGTCCGACGGTCATCGTCGCTGCCATTACGGCGCAGATTCAAAAGG
CGAAGCTGCCTACGCATGTGGAAATTGACGCAAAAACTTATGGATTTGATCGTGACTCCGTTATTCTGTTGGAACAAATA
CGAACCATTGACAAACAACGTCTCACTGATAAAATTACGCATCTCGATGACGAGATGATGGATCGGGTTAATGAATCCTT
GCAAATCAGTTTAGGACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11309.19 Da
• Isoelectric Point: 10.2101

>AT129203 WP_007721089.1 NZ_CP041767:525182-525481 [Brevibacillus brevis]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT129203 NZ_CP041767:525182-525481 [Brevibacillus brevis]
ATGGTTGGAGGTGGATTTGTCTTGTCGAAGTCAAACACGAAACGCATCATGATCAGCTTGCCACAACACTTGCTTCAGGA
AGTGGATGGCGTTATTCAAAAGGAAAAATCCAATCGCAGCGAGTTTATTCGCCAAGCGATGCATTTGTATTTGAAAGAGC
GAAAAAAACGCACAATCCGTGAGACCATGCAAAAAGGGTACATGGAGATGGCGAAGATCAATCTGAATATGGCATCGGAG
GCTTTTGGCGCTGAAGAAGAGGCTGACCATACTCTTGTCCGCTTAGTTAGCGGGGTGTGA

Structures

Toxin

• AlphaFold DB: J2PLP3

Antitoxin

• AlphaFold DB: J2GZH3