Detailed information of TA system TA129200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 80332..80896
• Replicon: chromosome
• Accession: NZ_CP041765
• Organism: Tomitella sp. HY188

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FO059_RS00360
• Protein ID: WP_168226635.1
• Coordinates: 80546..80896 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FO059_RS00355
• Protein ID: WP_143905405.1
• Coordinates: 80332..80562 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO059_RS00330	76003..76899	+	897	WP_143905395.1	MBL fold metallo-hydrolase	-
FO059_RS00335	76896..77846	+	951	WP_143905397.1	ABC transporter substrate-binding protein	-
FO059_RS00340	77843..78676	+	834	WP_143905399.1	ABC transporter permease	-
FO059_RS00345	78678..79436	+	759	WP_143905401.1	ABC transporter permease	-
FO059_RS00350	79433..80278	+	846	WP_143905403.1	ABC transporter ATP-binding protein	-
FO059_RS00355	80332..80562	+	231	WP_143905405.1	ribbon-helix-helix domain-containing protein	Antitoxin
FO059_RS00360	80546..80896	+	351	WP_168226635.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FO059_RS00365	80913..82046	-	1134	WP_143905409.1	RNA polymerase subunit sigma-24	-
FO059_RS00370	82046..82444	-	399	WP_143905411.1	hypothetical protein	-
FO059_RS00375	82620..83591	+	972	WP_143905413.1	zinc-binding dehydrogenase	-
FO059_RS00380	83661..85172	-	1512	WP_143905415.1	DHA2 family efflux MFS transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12569.65 Da
• Isoelectric Point: 11.3028

>T129200 WP_168226635.1 NZ_CP041765:80546-80896 [Tomitella sp. HY188]
MMLRGEVRLVNLEPARGSEANKKRPAVIVSNDRANATAARLGRGVVTVVLVTSSTARLYPFQVLLDSEVTGLRMDSKAQA
EQVRSVAVERIGPVIARLPAPTMNALDDALRLHLQL

Nucleotide

• Length: 351 bp

>T129200 NZ_CP041765:80546-80896 [Tomitella sp. HY188]
CTGATGCTGCGCGGTGAGGTGCGGCTGGTCAATCTGGAACCGGCTCGCGGAAGCGAGGCGAACAAGAAGCGTCCGGCGGT
CATCGTCAGCAATGACAGGGCCAACGCGACGGCGGCGCGCCTGGGCCGTGGTGTGGTGACGGTAGTGCTGGTGACCAGCA
GCACCGCCCGGCTGTATCCGTTCCAGGTTCTCCTCGACTCCGAGGTCACCGGGCTTCGTATGGATTCGAAGGCGCAGGCG
GAACAGGTCCGTTCAGTCGCGGTCGAACGTATCGGGCCGGTGATCGCACGTCTGCCTGCGCCGACGATGAATGCGCTCGA
CGACGCGCTCCGGTTGCACCTGCAACTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.00 Da
• Isoelectric Point: 3.9251

>AT129200 WP_143905405.1 NZ_CP041765:80332-80562 [Tomitella sp. HY188]
VKISVSLPEEDLEILDSYMRESGLGSRSAAIQRAVRMLRNVDLEDDYAAAWSEWESSGDAAAWESVAGDGLADAAR

Nucleotide

• Length: 231 bp

>AT129200 NZ_CP041765:80332-80562 [Tomitella sp. HY188]
GTGAAGATCAGCGTGAGCCTGCCGGAAGAGGACCTCGAGATTCTCGACTCGTACATGCGGGAATCCGGGCTGGGGTCACG
TTCCGCCGCAATCCAGCGGGCGGTCAGGATGCTCCGGAATGTCGATCTGGAAGACGATTACGCGGCGGCGTGGAGCGAGT
GGGAGTCGTCCGGCGACGCTGCCGCTTGGGAGTCGGTGGCGGGGGACGGTCTCGCTGATGCTGCGCGGTGA