Detailed information of TA system TA129166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518252..518888
• Replicon: chromosome
• Accession: NZ_CP041757
• Organism: Bacillus sp. KBS0812

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FFE90_RS02675
• Protein ID: WP_003156187.1
• Coordinates: 518538..518888 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: FFE90_RS02670
• Protein ID: WP_003225183.1
• Coordinates: 518252..518533 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFE90_RS02650	514611..515210	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
FFE90_RS02655	515305..515670	+	366	WP_003234281.1	holo-ACP synthase	-
FFE90_RS02660	515836..516852	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
FFE90_RS02665	516967..518136	+	1170	WP_003234284.1	alanine racemase	-
FFE90_RS02670	518252..518533	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FFE90_RS02675	518538..518888	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FFE90_RS02680	519003..519827	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
FFE90_RS02685	519832..520197	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
FFE90_RS02690	520201..520602	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
FFE90_RS02695	520614..521621	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
FFE90_RS02700	521683..522012	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
FFE90_RS02705	522009..522491	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
FFE90_RS02710	522457..523245	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
FFE90_RS02715	523245..523844	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T129166 WP_003156187.1 NZ_CP041757:518538-518888 [Bacillus sp. KBS0812]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T129166 NZ_CP041757:518538-518888 [Bacillus sp. KBS0812]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT129166 WP_003225183.1 NZ_CP041757:518252-518533 [Bacillus sp. KBS0812]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT129166 NZ_CP041757:518252-518533 [Bacillus sp. KBS0812]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32