Detailed information of TA system TA129164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 126001..126548
• Replicon: plasmid unnamed1
• Accession: NZ_CP041755
• Organism: Pseudomonas sp. KBS0707

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q48B48
• Locus tag: FFH21_RS29510
• Protein ID: WP_003319696.1
• Coordinates: 126001..126327 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: E7PGZ6
• Locus tag: FFH21_RS29515
• Protein ID: WP_004643921.1
• Coordinates: 126324..126548 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFH21_RS29470	121442..121861	-	420	WP_004661570.1	type II toxin-antitoxin system VapC family toxin	-
FFH21_RS29475	121858..122115	-	258	WP_003381346.1	Arc family DNA-binding protein	-
FFH21_RS29480	122580..123284	+	705	WP_004661990.1	ParA family protein	-
FFH21_RS29485	123281..123604	+	324	WP_004666942.1	hypothetical protein	-
FFH21_RS29490	123715..124074	+	360	Protein_131	SOS response-associated peptidase family protein	-
FFH21_RS29495	124027..124689	-	663	Protein_132	IS110 family transposase	-
FFH21_RS29500	124764..125663	+	900	Protein_133	IS3-like element ISPsy24 family transposase	-
FFH21_RS29505	125674..125883	-	210	Protein_134	hypothetical protein	-
FFH21_RS29510	126001..126327	-	327	WP_003319696.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FFH21_RS29515	126324..126548	-	225	WP_004643921.1	antitoxin MazE family protein	Antitoxin
FFH21_RS29520	126886..127307	+	422	Protein_137	transposase	-
FFH21_RS29530	127527..129302	+	1776	Protein_138	conjugal transfer protein	-
FFH21_RS29540	129542..129790	+	249	Protein_139	type IV secretion system protein	-
FFH21_RS29545	129780..130313	+	534	Protein_140	TrbG/VirB9 family P-type conjugative transfer protein	-
FFH21_RS29550	130434..131084	+	651	WP_005730296.1	AAA family ATPase	-
FFH21_RS29555	131075..131428	+	354	WP_005730297.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131974	131974
	-	inside	IScluster/Tn	-	-	124749..127164	2415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (8-72)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11660.71 Da
• Isoelectric Point: 8.0546

>T129164 WP_003319696.1 NZ_CP041755:c126327-126001 [Pseudomonas sp. KBS0707]
MMRGDLVTVAMQGDFGKPRPALVIQANQFSEHTSVTVLPVTSTLVAAPLLRITVQPTAENGLQKPSQVMLDKTMTLKREK
IGPTFGHIGVDSMVEVERCLAVFLGIAN

Nucleotide

• Length: 327 bp

>T129164 NZ_CP041755:c126327-126001 [Pseudomonas sp. KBS0707]
ATGATGCGAGGTGACCTAGTCACCGTCGCCATGCAAGGTGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCAAA
CCAATTTAGCGAACACACCAGCGTTACCGTGCTTCCAGTGACCAGCACCCTAGTCGCAGCACCGCTGCTGCGCATCACCG
TTCAGCCGACCGCAGAAAACGGCTTGCAGAAGCCTTCTCAGGTCATGCTAGACAAGACCATGACCCTCAAGCGTGAAAAA
ATAGGCCCGACTTTTGGGCATATCGGAGTGGATTCAATGGTGGAGGTGGAGCGCTGTTTAGCCGTGTTCTTGGGTATCGC
CAATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8501.64 Da
• Isoelectric Point: 6.2008

>AT129164 WP_004643921.1 NZ_CP041755:c126548-126324 [Pseudomonas sp. KBS0707]
MPTSHVNTRVQRHRDALRAAGLRPIQIWVPDTRRPDFAQECHRQCLLVAQADMANTYMQGLMDEALDDVEGWTP

Nucleotide

• Length: 225 bp

>AT129164 NZ_CP041755:c126548-126324 [Pseudomonas sp. KBS0707]
ATGCCTACATCCCATGTAAATACCCGCGTTCAACGCCATCGCGACGCGCTGCGTGCGGCTGGCCTACGGCCCATTCAGAT
TTGGGTTCCCGATACCCGGCGGCCAGACTTTGCACAGGAGTGCCATCGACAGTGCCTGTTGGTTGCTCAGGCAGACATGG
CCAATACCTATATGCAGGGGTTGATGGACGAAGCCTTAGACGATGTTGAGGGTTGGACGCCATGA

Structures

Toxin

• AlphaFold DB: A0A7V8S2J7

Antitoxin

• AlphaFold DB: A0A0P9S763