Detailed information of TA system TA129163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 121442..122115
• Replicon: plasmid unnamed1
• Accession: NZ_CP041755
• Organism: Pseudomonas sp. KBS0707

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q48B53
• Locus tag: FFH21_RS29470
• Protein ID: WP_004661570.1
• Coordinates: 121442..121861 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q48B52
• Locus tag: FFH21_RS29475
• Protein ID: WP_003381346.1
• Coordinates: 121858..122115 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFH21_RS29465	120769..121401	-	633	WP_004666965.1	recombinase family protein	-
FFH21_RS29470	121442..121861	-	420	WP_004661570.1	type II toxin-antitoxin system VapC family toxin	Toxin
FFH21_RS29475	121858..122115	-	258	WP_003381346.1	Arc family DNA-binding protein	Antitoxin
FFH21_RS29480	122580..123284	+	705	WP_004661990.1	ParA family protein	-
FFH21_RS29485	123281..123604	+	324	WP_004666942.1	hypothetical protein	-
FFH21_RS29490	123715..124074	+	360	Protein_131	SOS response-associated peptidase family protein	-
FFH21_RS29495	124027..124689	-	663	Protein_132	IS110 family transposase	-
FFH21_RS29500	124764..125663	+	900	Protein_133	IS3-like element ISPsy24 family transposase	-
FFH21_RS29505	125674..125883	-	210	Protein_134	hypothetical protein	-
FFH21_RS29510	126001..126327	-	327	WP_003319696.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FFH21_RS29515	126324..126548	-	225	WP_004643921.1	antitoxin MazE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131974	131974
	-	inside	IScluster/Tn	-	-	124749..127164	2415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15137.44 Da
• Isoelectric Point: 4.8781

>T129163 WP_004661570.1 NZ_CP041755:c121861-121442 [Pseudomonas sp. KBS0707]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRNTGPFEAAGLNVINPWEA

Nucleotide

• Length: 420 bp

>T129163 NZ_CP041755:c121861-121442 [Pseudomonas sp. KBS0707]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCTGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTACGTGAAAACCTGGAAAAGCACTTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGGCTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTTGCCACCCGAAACACTGGCCCTTTCGAGGCTGCCGGGTTGAATGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9401.62 Da
• Isoelectric Point: 9.0330

>AT129163 WP_003381346.1 NZ_CP041755:c122115-121858 [Pseudomonas sp. KBS0707]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGSFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Nucleotide

• Length: 258 bp

>AT129163 NZ_CP041755:c122115-121858 [Pseudomonas sp. KBS0707]
GTGGCAAACGTGAACGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Structures

Toxin

• AlphaFold DB: Q48B53

Antitoxin

• AlphaFold DB: Q48B52