Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapB(antitoxin)
Location 29038..29612 Replicon plasmid unnamed1
Accession NZ_CP041755
Organism Pseudomonas sp. KBS0707

Toxin (Protein)


Gene name vapC Uniprot ID Q48BC5
Locus tag FFH21_RS28885 Protein ID WP_003344395.1
Coordinates 29038..29415 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q48BC4
Locus tag FFH21_RS28890 Protein ID WP_004644067.1
Coordinates 29412..29612 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFH21_RS28855 24751..25266 + 516 Protein_28 IS110-like element ISPsy16 family transposase -
FFH21_RS28870 26028..28298 + 2271 WP_032704248.1 hypothetical protein -
FFH21_RS28875 28301..28471 - 171 Protein_30 transposase -
FFH21_RS28880 28704..29048 + 345 Protein_31 conjugal transfer protein -
FFH21_RS28885 29038..29415 - 378 WP_003344395.1 PIN domain-containing protein Toxin
FFH21_RS28890 29412..29612 - 201 WP_004644067.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FFH21_RS28895 29704..30384 + 681 Protein_34 hypothetical protein -
FFH21_RS28900 30371..30640 + 270 Protein_35 type IV secretory system conjugative DNA transfer family protein -
FFH21_RS28910 30898..32130 - 1233 Protein_36 IS91 family transposase -
FFH21_RS28920 32342..32572 + 231 WP_019331426.1 hypothetical protein -
FFH21_RS28925 32662..33201 + 540 WP_050772298.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..131974 131974
- flank IS/Tn - - 24769..25266 497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13633.73 Da        Isoelectric Point: 6.4723

>T129160 WP_003344395.1 NZ_CP041755:c29415-29038 [Pseudomonas sp. KBS0707]
VKGVLVDTSVWVEHFRNNSPELVNLLSQDRVLIHPMVIGELACGTPPDRSNTLTDLGDLRGAQQPTVSEVIAFLNTHKLY
GLGCGLVDMTLLASALLSGTALWTLDKRLERLASRMAVSYQPPTH

Download         Length: 378 bp

>T129160 NZ_CP041755:c29415-29038 [Pseudomonas sp. KBS0707]
GTGAAAGGCGTCCTGGTAGACACCTCCGTTTGGGTTGAGCACTTTCGCAACAACAGTCCAGAACTGGTGAACTTGCTCAG
CCAGGACCGGGTACTCATACACCCCATGGTGATCGGAGAACTGGCGTGTGGTACGCCGCCTGATCGTTCCAACACCTTGA
CCGACCTTGGCGACCTACGAGGTGCACAACAACCCACGGTGAGTGAAGTCATAGCCTTTCTGAACACCCACAAGCTCTAT
GGCCTGGGCTGTGGACTGGTGGATATGACGCTCCTGGCCTCCGCGTTATTGAGCGGCACAGCGCTTTGGACGTTAGACAA
GCGCCTGGAGCGTTTGGCTAGCCGCATGGCCGTGTCATACCAGCCTCCTACTCACTAG

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7445.66 Da        Isoelectric Point: 7.0102

>AT129160 WP_004644067.1 NZ_CP041755:c29612-29412 [Pseudomonas sp. KBS0707]
MRTTVTIDDALYQRALEVADPAMDKADLFREAVQTFVRIQAAKRLMALGATLPTMEDIARRHEKAL

Download         Length: 201 bp

>AT129160 NZ_CP041755:c29612-29412 [Pseudomonas sp. KBS0707]
ATGAGAACGACCGTCACCATCGACGATGCGTTGTACCAACGTGCTCTGGAAGTCGCCGATCCGGCGATGGACAAGGCCGA
CCTGTTCCGTGAAGCCGTTCAGACGTTTGTGCGCATTCAAGCGGCTAAACGATTGATGGCTTTAGGGGCCACACTGCCAA
CGATGGAAGACATCGCCCGCCGACACGAGAAGGCACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3GPE2

Antitoxin

Source ID Structure
AlphaFold DB A0A2S4IKB9

References