Detailed information of TA system TA129151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 545054..545933
• Replicon: chromosome
• Accession: NZ_CP041753
• Organism: Pseudomonas sp. ATCC 43928

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FFH90_RS02480
• Protein ID: WP_168206151.1
• Coordinates: 545054..545407 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFH90_RS02485
• Protein ID: WP_138866642.1
• Coordinates: 545460..545933 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFH90_RS02460	540322..541989	-	1668	WP_138866826.1	DUF262 domain-containing HNH endonuclease family protein	-
FFH90_RS02470	542652..542882	+	231	WP_138866640.1	hypothetical protein	-
FFH90_RS02475	543414..544673	-	1260	WP_138866641.1	DUF5623 domain-containing protein	-
FFH90_RS02480	545054..545407	+	354	WP_168206151.1	type II toxin-antitoxin system HigB family toxin	Toxin
FFH90_RS02485	545460..545933	+	474	WP_138866642.1	transcriptional regulator	Antitoxin
FFH90_RS02490	546371..547984	-	1614	WP_138866643.1	TIGR04141 family sporadically distributed protein	-
FFH90_RS02500	548531..549520	-	990	WP_138866645.1	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	539208..547984	8776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (70-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13674.76 Da
• Isoelectric Point: 10.0709

>T129151 WP_168206151.1 NZ_CP041753:545054-545407 [Pseudomonas sp. ATCC 43928]
MRVIAKTAVAQAIEAHSQWKDPLSLWLTTFDRATLRFESFEQLRETWATASGWNVDRIPRSKLRHTSLKGPLDIYVFDIK
KNECRIISWINPRNGTIYIKDILPHAGYDKWCKSDVK

Nucleotide

• Length: 354 bp

>T129151 NZ_CP041753:545054-545407 [Pseudomonas sp. ATCC 43928]
ATGCGAGTGATAGCAAAAACGGCGGTTGCACAAGCCATTGAAGCCCATAGCCAGTGGAAAGACCCACTGAGCTTGTGGCT
AACCACCTTCGACAGGGCGACCCTTCGCTTTGAGTCCTTTGAGCAGCTTCGAGAAACCTGGGCCACCGCTTCTGGATGGA
ACGTCGATCGAATCCCTCGCTCAAAGCTAAGGCATACAAGCCTTAAAGGGCCTCTTGATATATATGTCTTCGATATCAAG
AAAAATGAGTGTCGGATTATTTCGTGGATTAACCCGCGAAATGGCACCATTTATATAAAGGACATTCTGCCTCACGCTGG
ATATGACAAGTGGTGCAAGAGTGACGTTAAGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17473.75 Da
• Isoelectric Point: 4.4307

>AT129151 WP_138866642.1 NZ_CP041753:545460-545933 [Pseudomonas sp. ATCC 43928]
MKHDKIEATGLDSFIANISPMLDGLSDQIATMSQLLSACHDPIKNDDDLQGRMALIDELYSHADSEDHAAARFAELVADR
VYEYETETVLIPYSSQSEALAFLISDRGVKQKDLSDIATQSAVSEILNNKRKMTVAQIKGFSEFFKVPVEFFMHGIV

Nucleotide

• Length: 474 bp

>AT129151 NZ_CP041753:545460-545933 [Pseudomonas sp. ATCC 43928]
ATGAAGCATGATAAGATCGAAGCCACTGGACTAGATTCCTTCATAGCTAATATTTCACCAATGCTTGATGGTCTAAGCGA
TCAGATAGCTACGATGTCCCAGTTGTTATCTGCATGTCATGATCCAATTAAAAATGATGATGACTTGCAGGGACGGATGG
CGCTAATTGATGAGCTTTACTCTCATGCAGATAGTGAAGATCACGCAGCCGCACGCTTTGCGGAGTTGGTTGCTGATCGC
GTGTATGAGTACGAGACTGAAACAGTTTTGATTCCTTACTCCTCTCAGTCGGAAGCACTAGCTTTCCTCATTTCGGATAG
GGGAGTTAAGCAAAAGGATTTGTCTGATATAGCAACTCAAAGTGCTGTTTCTGAAATACTAAATAATAAGAGAAAAATGA
CTGTCGCTCAAATAAAAGGCTTTTCTGAATTTTTTAAAGTTCCAGTCGAGTTTTTTATGCATGGCATAGTCTGA