Detailed information of TA system TA129138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4248428..4248954
• Replicon: chromosome
• Accession: NZ_CP041749
• Organism: Escherichia coli strain NCCP 15955

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FOV95_RS20755
• Protein ID: WP_000323025.1
• Coordinates: 4248667..4248954 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FOV95_RS20750
• Protein ID: WP_000534858.1
• Coordinates: 4248428..4248667 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOV95_RS20725	4243755..4244105	+	351	WP_000624701.1	IS66 family insertion sequence element accessory protein TnpB	-
FOV95_RS20730	4244136..4245749	+	1614	WP_044186286.1	IS66-like element IS682 family transposase	-
FOV95_RS20735	4246227..4246559	-	333	WP_001301033.1	protein FlxA	-
FOV95_RS20745	4248131..4248403	-	273	WP_072033201.1	hypothetical protein	-
FOV95_RS20750	4248428..4248667	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FOV95_RS20755	4248667..4248954	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FOV95_RS20760	4249026..4249181	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
FOV95_RS20765	4249377..4250489	-	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
FOV95_RS20770	4250747..4250998	+	252	WP_000980994.1	hypothetical protein	-
FOV95_RS20775	4251065..4251343	+	279	WP_012304870.1	hypothetical protein	-
FOV95_RS20780	4251345..4252394	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
FOV95_RS20785	4252408..4253160	+	753	WP_001047135.1	antitermination protein	-
FOV95_RS20790	4253438..4253527	-	90	WP_120795389.1	hypothetical protein	-
FOV95_RS20795	4253582..4253794	-	213	WP_000087756.1	cold shock-like protein CspF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stxA / stxB	4146555..4265618	119063
	-	inside	IScluster/Tn	-	-	4236873..4250489	13616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T129138 WP_000323025.1 NZ_CP041749:4248667-4248954 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T129138 NZ_CP041749:4248667-4248954 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT129138 WP_000534858.1 NZ_CP041749:4248428-4248667 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT129138 NZ_CP041749:4248428-4248667 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8