Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3041516..3042134 Replicon chromosome
Accession NZ_CP041749
Organism Escherichia coli strain NCCP 15955

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FOV95_RS14670 Protein ID WP_001291435.1
Coordinates 3041516..3041734 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FOV95_RS14675 Protein ID WP_000344800.1
Coordinates 3041760..3042134 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOV95_RS14635 3036806..3037378 + 573 WP_000779831.1 YbaY family lipoprotein -
FOV95_RS14640 3037409..3037720 - 312 WP_000409911.1 MGMT family protein -
FOV95_RS14650 3038099..3038452 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FOV95_RS14655 3038494..3040043 - 1550 Protein_2844 cyclic-guanylate-specific phosphodiesterase PdeB -
FOV95_RS14660 3040207..3040677 - 471 WP_000136192.1 YlaC family protein -
FOV95_RS14665 3040793..3041344 - 552 WP_000102564.1 maltose O-acetyltransferase -
FOV95_RS14670 3041516..3041734 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FOV95_RS14675 3041760..3042134 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FOV95_RS14680 3042680..3045829 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FOV95_RS14685 3045852..3047045 - 1194 WP_033801653.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T129135 WP_001291435.1 NZ_CP041749:c3041734-3041516 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T129135 NZ_CP041749:c3041734-3041516 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT129135 WP_000344800.1 NZ_CP041749:c3042134-3041760 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT129135 NZ_CP041749:c3042134-3041760 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References