Detailed information of TA system TA129129

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1574777..1575612
• Replicon: chromosome
• Accession: NZ_CP041749
• Organism: Escherichia coli strain NCCP 15955

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2A2CEQ6
• Locus tag: FOV95_RS07735
• Protein ID: WP_032261156.1
• Coordinates: 1575235..1575612 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2A2CE58
• Locus tag: FOV95_RS07730
• Protein ID: WP_032260920.1
• Coordinates: 1574777..1575145 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOV95_RS07695	1570501..1571181	+	681	WP_001282919.1	WYL domain-containing protein	-
FOV95_RS07700	1571329..1572006	+	678	WP_033801393.1	hypothetical protein	-
FOV95_RS07705	1572012..1572245	+	234	WP_033801392.1	DUF905 family protein	-
FOV95_RS07710	1572335..1573153	+	819	WP_033802047.1	DUF945 domain-containing protein	-
FOV95_RS07715	1573419..1573898	+	480	WP_000706975.1	antirestriction protein	-
FOV95_RS07720	1573913..1574389	+	477	WP_001318130.1	RadC family protein	-
FOV95_RS07725	1574476..1574697	+	222	WP_000692350.1	DUF987 domain-containing protein	-
FOV95_RS07730	1574777..1575145	+	369	WP_032260920.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FOV95_RS07735	1575235..1575612	+	378	WP_032261156.1	TA system toxin CbtA family protein	Toxin
FOV95_RS07740	1575609..1575758	+	150	Protein_1510	hypothetical protein	-
FOV95_RS07745	1575834..1576031	+	198	WP_086795284.1	DUF957 domain-containing protein	-
FOV95_RS07750	1576116..1576958	+	843	WP_001521669.1	DUF4942 domain-containing protein	-
FOV95_RS07760	1577754..1578938	+	1185	WP_001172876.1	sugar efflux transporter	-
FOV95_RS07765	1579049..1579972	+	924	WP_000535961.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14080.04 Da
• Isoelectric Point: 7.2919

>T129129 WP_032261156.1 NZ_CP041749:1575235-1575612 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRMVNDISQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T129129 NZ_CP041749:1575235-1575612 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATGACATTAGCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13733.58 Da
• Isoelectric Point: 6.4669

>AT129129 WP_032260920.1 NZ_CP041749:1574777-1575145 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYVKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT129129 NZ_CP041749:1574777-1575145 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCAGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGTAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2A2CEQ6

Antitoxin

• AlphaFold DB: A0A2A2CE58