Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 589137..589791 Replicon chromosome
Accession NZ_CP041749
Organism Escherichia coli strain NCCP 15955

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FOV95_RS02965 Protein ID WP_000244777.1
Coordinates 589137..589544 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FOV95_RS02970 Protein ID WP_000354046.1
Coordinates 589525..589791 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOV95_RS02945 585095..586828 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
FOV95_RS02950 586834..587543 - 710 Protein_564 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FOV95_RS02955 587568..588464 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FOV95_RS02960 588576..589097 + 522 WP_001055874.1 flavodoxin FldB -
FOV95_RS02965 589137..589544 - 408 WP_000244777.1 toxin CptA Toxin
FOV95_RS02970 589525..589791 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FOV95_RS02975 590034..591014 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FOV95_RS02980 591210..591869 - 660 WP_000250274.1 hemolysin III family protein -
FOV95_RS02985 592033..592344 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
FOV95_RS02990 592389..593822 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
FOV95_RS02995 593879..594622 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T129124 WP_000244777.1 NZ_CP041749:c589544-589137 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T129124 NZ_CP041749:c589544-589137 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT129124 WP_000354046.1 NZ_CP041749:c589791-589525 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT129124 NZ_CP041749:c589791-589525 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References