Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 36840..37411 Replicon plasmid p2
Accession NZ_CP041740
Organism Enterococcus faecalis EnGen0107 strain B594

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z2CGM4
Locus tag SAW_RS17250 Protein ID WP_002362432.1
Coordinates 37070..37411 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag SAW_RS17245 Protein ID WP_002362431.1
Coordinates 36840..37070 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAW_RS17220 32714..32989 + 276 WP_001196543.1 hypothetical protein -
SAW_RS17225 33217..33897 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
SAW_RS17230 34025..34984 + 960 WP_000222577.1 IS30-like element IS1252 family transposase -
SAW_RS17235 35417..35989 - 573 WP_000170424.1 recombinase family protein -
SAW_RS17240 36005..36610 - 606 WP_000599739.1 cell filamentation protein -
SAW_RS17245 36840..37070 + 231 WP_002362431.1 hypothetical protein Antitoxin
SAW_RS17250 37070..37411 + 342 WP_002362432.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SAW_RS17255 37523..37993 + 471 Protein_45 hypothetical protein -
SAW_RS17260 38048..38728 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
SAW_RS17265 38782..39762 - 981 WP_010714235.1 hypothetical protein -
SAW_RS17270 39762..40379 - 618 WP_010714234.1 recombinase family protein -
SAW_RS17425 40393..40563 - 171 WP_162838404.1 hypothetical protein -
SAW_RS17275 40912..42402 - 1491 WP_010714232.1 primase C-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-aph(2'') / aadD / erm(B) - 1..45436 45436
- inside IScluster/Tn erm(B) - 33211..44135 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13244.44 Da        Isoelectric Point: 8.0113

>T129115 WP_002362432.1 NZ_CP041740:37070-37411 [Enterococcus faecalis EnGen0107]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T129115 NZ_CP041740:37070-37411 [Enterococcus faecalis EnGen0107]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT129115 WP_002362431.1 NZ_CP041740:36840-37070 [Enterococcus faecalis EnGen0107]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT129115 NZ_CP041740:36840-37070 [Enterococcus faecalis EnGen0107]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2CGM4

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References