Detailed information of TA system TA129090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2729795..2730828
• Replicon: chromosome
• Accession: NZ_CP041738
• Organism: Enterococcus faecalis EnGen0107 strain B594

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A855UI70
• Locus tag: SAW_RS14440
• Protein ID: WP_002399531.1
• Coordinates: 2730175..2730828 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: SAW_RS14435
• Protein ID: WP_002399530.1
• Coordinates: 2729795..2730178 (+)
• Length: 128 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAW_RS14385	2725620..2725988	-	369	WP_002365216.1	DUF86 domain-containing protein	-
SAW_RS14390	2725972..2726304	-	333	WP_002399122.1	nucleotidyltransferase domain-containing protein	-
SAW_RS14395	2726364..2726687	-	324	WP_002399121.1	hypothetical protein	-
SAW_RS14400	2726727..2726966	-	240	WP_002399524.1	hypothetical protein	-
SAW_RS14405	2726977..2727129	-	153	WP_002399525.1	hypothetical protein	-
SAW_RS14410	2727156..2727380	-	225	WP_002365222.1	hypothetical protein	-
SAW_RS14415	2727612..2727800	+	189	WP_002399526.1	hypothetical protein	-
SAW_RS17400	2727787..2727948	-	162	WP_002399528.1	hypothetical protein	-
SAW_RS14420	2727971..2728387	-	417	WP_002380876.1	DUF961 family protein	-
SAW_RS14425	2728387..2728713	-	327	WP_002365226.1	hypothetical protein	-
SAW_RS14430	2728799..2729050	-	252	WP_002399529.1	hypothetical protein	-
SAW_RS14435	2729795..2730178	+	384	WP_002399530.1	helix-turn-helix transcriptional regulator	Antitoxin
SAW_RS14440	2730175..2730828	+	654	WP_002399531.1	ImmA/IrrE family metallo-endopeptidase	Toxin
SAW_RS14445	2730867..2731202	+	336	WP_002399532.1	helix-turn-helix transcriptional regulator	-
SAW_RS14450	2731240..2732571	-	1332	WP_002365232.1	FAD-dependent oxidoreductase	-
SAW_RS14455	2732670..2733605	-	936	WP_171803825.1	aldo/keto reductase	-
SAW_RS14460	2733620..2734039	-	420	WP_002371441.1	MerR family transcriptional regulator	-
SAW_RS14465	2734056..2734637	-	582	WP_002380883.1	phosphoglycerate mutase family protein	-
SAW_RS14470	2734859..2735188	+	330	WP_002365236.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2665032..2764919	99887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (80-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26078.10 Da
• Isoelectric Point: 6.7561

>T129090 WP_002399531.1 NZ_CP041738:2730175-2730828 [Enterococcus faecalis EnGen0107]
MIGLLLVDFISKKLYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEEVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEVMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKKF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T129090 NZ_CP041738:2730175-2730828 [Enterococcus faecalis EnGen0107]
TTGATTGGACTGTTGTTGGTGGATTTTATTAGTAAAAAACTCTATTTTGAAGCATTTAATTTTTCAAACGAATTAATAAA
AGAAGTATCTTACTATTCTTCCAAAAATATTGAACAAGTTAAATGCTTTGATATTGAAAAATATGTAAAAGAAGTTGAAG
AAGTTGAATTTGTTGAATACACGTTTCAAAAACAACTTAAAAGACGGATGCTTGGTTCAATTTCTAAAGTAGACGATGAA
GTCATTATAACAACAAACAAAGAATTAATGTTAGAACGAAAAAATTTCACTAAAATGCATGAGGTTATGCATTACTACAT
AGATATACCTAAAATTAATAATGCAACTCATACTTTTTCTGATATTCTTTTAAAAAATGGTTACTTGATGGAAGATTTTC
CAAAAGAATATCGTGCTAATGTCGGTGCTAGCATGTTAATGGCAAATGACCAAGCATTGTTTTACGCATTAAAAAAATTT
TATTCATTCGAAGAAATTGCACAATACTTTTTTATGAGCAAATCTGCATTAAGAAATAGACTCATCGAGCATCTTATGTA
TGTAAATAACTGTACTTTTGCACACGCTAATACACTTTTTAACAATTATTACTTTCATAAAGAAACTGATATCTACAGAT
TTATTTTCAATTAG

Antitoxin

Protein

• Length: 128 a.a.
• Molecular weight: 15017.02 Da
• Isoelectric Point: 5.3634

>AT129090 WP_002399530.1 NZ_CP041738:2729795-2730178 [Enterococcus faecalis EnGen0107]
VQPLAKRLQLLREEKEWTKTYVAKQLGLNNLGTYANWEYGTREPDSEMLSKIASLYDVSTDFLLGRTEERIHLNDNEKIG
KNIISHFRLNTSDMNIEDIEELEEELIDFQDFLIKKAKEKKKRNKKD

Nucleotide

• Length: 384 bp

>AT129090 NZ_CP041738:2729795-2730178 [Enterococcus faecalis EnGen0107]
GTGCAACCATTAGCAAAAAGACTACAGCTTTTAAGAGAAGAAAAAGAATGGACAAAAACATATGTTGCAAAACAACTAGG
TTTAAATAACTTAGGAACCTATGCAAACTGGGAATATGGAACACGTGAACCGGATTCGGAAATGCTATCAAAAATAGCTT
CCCTATATGATGTATCTACCGATTTTTTACTAGGTAGAACAGAGGAAAGAATACATCTAAACGACAATGAAAAAATTGGA
AAAAATATCATTTCTCATTTTCGTTTAAATACTTCTGATATGAACATAGAAGATATAGAAGAATTAGAAGAAGAATTAAT
CGATTTTCAAGATTTTCTTATAAAAAAAGCAAAAGAAAAAAAGAAACGTAACAAAAAAGATTGA

Structures

Toxin

• AlphaFold DB: A0A855UI70