Detailed information of TA system TA129088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 442173..442707
• Replicon: chromosome
• Accession: NZ_CP041738
• Organism: Enterococcus faecalis EnGen0107 strain B594

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: SAW_RS02485
• Protein ID: WP_002360769.1
• Coordinates: 442432..442707 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: SAW_RS02480
• Protein ID: WP_002369771.1
• Coordinates: 442173..442439 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAW_RS02445	438031..438816	+	786	WP_002360778.1	replication-relaxation family protein	-
SAW_RS02450	438822..439310	+	489	WP_010710133.1	hypothetical protein	-
SAW_RS02455	439902..440168	+	267	WP_002377947.1	hypothetical protein	-
SAW_RS02460	440201..440701	+	501	WP_002360775.1	DnaJ domain-containing protein	-
SAW_RS02465	440714..440962	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
SAW_RS02470	441038..441454	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
SAW_RS02475	441478..442062	+	585	WP_002377949.1	thermonuclease family protein	-
SAW_RS02480	442173..442439	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SAW_RS02485	442432..442707	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SAW_RS02490	442898..443578	-	681	Protein_434	IS6-like element IS1216 family transposase	-
SAW_RS02495	443663..444557	+	895	Protein_435	IS256 family transposase	-
SAW_RS02500	444862..445899	-	1038	WP_002355369.1	PTS sugar transporter subunit IIC	-
SAW_RS02505	445925..446863	-	939	WP_002370935.1	2-dehydropantoate 2-reductase	-
SAW_RS02510	446991..447320	-	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
SAW_RS02515	447350..447535	+	186	WP_002358660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / esp	418277..481575	63298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T129088 WP_002360769.1 NZ_CP041738:442432-442707 [Enterococcus faecalis EnGen0107]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T129088 NZ_CP041738:442432-442707 [Enterococcus faecalis EnGen0107]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT129088 WP_002369771.1 NZ_CP041738:442173-442439 [Enterococcus faecalis EnGen0107]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT129088 NZ_CP041738:442173-442439 [Enterococcus faecalis EnGen0107]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7