Detailed information of TA system TA129085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 218485..219011
• Replicon: plasmid pME-1a
• Accession: NZ_CP041734
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain ME-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: FO617_RS24560
• Protein ID: WP_000323025.1
• Coordinates: 218485..218772 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: FO617_RS24565
• Protein ID: WP_000534858.1
• Coordinates: 218772..219011 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO617_RS25135	214055..214231	-	177	WP_001371930.1	hypothetical protein	-
FO617_RS24530	214742..215686	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
FO617_RS24535	215785..216384	+	600	WP_001031420.1	hypothetical protein	-
FO617_RS24540	216444..216794	+	351	WP_000743059.1	hypothetical protein	-
FO617_RS24545	216841..217044	+	204	WP_001015183.1	hypothetical protein	-
FO617_RS24550	217326..217646	+	321	WP_000332796.1	hypothetical protein	-
FO617_RS24555	218255..218413	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
FO617_RS24560	218485..218772	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FO617_RS24565	218772..219011	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
FO617_RS24575	219274..220197	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
FO617_RS24580	220397..220969	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
FO617_RS24590	221445..222683	-	1239	WP_000219087.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaVIM-4 / aac(6')-Il / dfrA1 / aadA1 / qacE / sul1 / qnrA1 / dfrA16 / aadA2 / blaCTX-M-9 / mcr-9	-	1..276520	276520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T129085 WP_000323025.1 NZ_CP041734:c218772-218485 [Enterobacter hormaechei subsp. steigerwaltii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T129085 NZ_CP041734:c218772-218485 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT129085 WP_000534858.1 NZ_CP041734:c219011-218772 [Enterobacter hormaechei subsp. steigerwaltii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT129085 NZ_CP041734:c219011-218772 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8