Detailed information of TA system TA129083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4715909..4716525
• Replicon: chromosome
• Accession: NZ_CP041733
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain ME-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FO617_RS22945
• Protein ID: WP_017382676.1
• Coordinates: 4715909..4716280 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: FO617_RS22950
• Protein ID: WP_015569912.1
• Coordinates: 4716283..4716525 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO617_RS22930	4713409..4714311	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
FO617_RS22935	4714308..4714943	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
FO617_RS22940	4714940..4715869	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
FO617_RS22945	4715909..4716280	-	372	WP_017382676.1	type II toxin-antitoxin system VapC family toxin	Toxin
FO617_RS22950	4716283..4716525	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
FO617_RS22955	4716724..4717644	+	921	WP_063158447.1	alpha/beta hydrolase	-
FO617_RS22960	4717653..4718594	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
FO617_RS22965	4718639..4719076	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FO617_RS22970	4719073..4719954	-	882	WP_003861949.1	virulence factor BrkB family protein	-
FO617_RS22975	4719948..4720547	-	600	WP_003861947.1	glucose-1-phosphatase	-
FO617_RS22980	4720666..4721466	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T129083 WP_017382676.1 NZ_CP041733:c4716280-4715909 [Enterobacter hormaechei subsp. steigerwaltii]
MEHMAVFDTNILIDLFNNRIEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T129083 NZ_CP041733:c4716280-4715909 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTATTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT129083 WP_015569912.1 NZ_CP041733:c4716525-4716283 [Enterobacter hormaechei subsp. steigerwaltii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT129083 NZ_CP041733:c4716525-4716283 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATTGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAGCCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3