Detailed information of TA system TA129071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2399443..2400182
• Replicon: chromosome
• Accession: NZ_CP041733
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain ME-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: FO617_RS11900
• Protein ID: WP_144427872.1
• Coordinates: 2399443..2399928 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: FO617_RS11905
• Protein ID: WP_003857131.1
• Coordinates: 2399916..2400182 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO617_RS11880	2395064..2395504	-	441	WP_016239970.1	hypothetical protein	-
FO617_RS11885	2395657..2397018	-	1362	WP_000482601.1	FRG domain-containing protein	-
FO617_RS11890	2397032..2397835	-	804	WP_000589340.1	hypothetical protein	-
FO617_RS11895	2397894..2399240	-	1347	WP_076611902.1	ISNCY-like element ISLad2 family transposase	-
FO617_RS11900	2399443..2399928	-	486	WP_144427872.1	GNAT family N-acetyltransferase	Toxin
FO617_RS11905	2399916..2400182	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
FO617_RS11910	2400246..2401175	-	930	WP_144427873.1	LysR family transcriptional regulator	-
FO617_RS11915	2401305..2402687	+	1383	WP_144428050.1	MFS transporter	-
FO617_RS11920	2402709..2403704	-	996	WP_144427874.1	DUF2891 domain-containing protein	-
FO617_RS11925	2403714..2404700	-	987	WP_017382341.1	DUF979 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2383227..2400182	16955
	-	flank	IS/Tn	-	-	2397894..2399252	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17570.26 Da
• Isoelectric Point: 9.9658

>T129071 WP_144427872.1 NZ_CP041733:c2399928-2399443 [Enterobacter hormaechei subsp. steigerwaltii]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTQATGNLRRNM
PDPIPVIILTRLAVDVSLRGNGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T129071 NZ_CP041733:c2399928-2399443 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTACCCGTCTGGCTGTCGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCCGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT129071 WP_003857131.1 NZ_CP041733:c2400182-2399916 [Enterobacter hormaechei subsp. steigerwaltii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT129071 NZ_CP041733:c2400182-2399916 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACAGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCACAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9