Detailed information of TA system TA129068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 295754..296350
• Replicon: chromosome
• Accession: NZ_CP041733
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain ME-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A837F9W8
• Locus tag: FO617_RS01425
• Protein ID: WP_023315213.1
• Coordinates: 296048..296350 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: FO617_RS01420
• Protein ID: WP_032672198.1
• Coordinates: 295754..296041 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO617_RS01415	294126..295757	+	1632	WP_003860350.1	Na/Pi cotransporter family protein	-
FO617_RS01420	295754..296041	-	288	WP_032672198.1	putative addiction module antidote protein	Antitoxin
FO617_RS01425	296048..296350	-	303	WP_023315213.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FO617_RS01430	296548..297420	+	873	WP_047726112.1	23S rRNA pseudouridine(2604) synthase RluF	-
FO617_RS01435	297421..297693	-	273	WP_017382612.1	DUF3811 domain-containing protein	-
FO617_RS01440	297744..298688	-	945	WP_144427628.1	ketopantoate/pantoate/pantothenate transporter PanS	-
FO617_RS01445	298782..300131	-	1350	WP_006810501.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11427.21 Da
• Isoelectric Point: 10.1042

>T129068 WP_023315213.1 NZ_CP041733:c296350-296048 [Enterobacter hormaechei subsp. steigerwaltii]
MKEIVQTESFRRWEQNLKDRRAKTIIASRLFRLANGLAGDIRPVGEGISELRIHFGPGYRVYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Nucleotide

• Length: 303 bp

>T129068 NZ_CP041733:c296350-296048 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10088.51 Da
• Isoelectric Point: 4.7045

>AT129068 WP_032672198.1 NZ_CP041733:c296041-295754 [Enterobacter hormaechei subsp. steigerwaltii]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAEALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPSGD

Nucleotide

• Length: 288 bp

>AT129068 NZ_CP041733:c296041-295754 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTGAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: A0A837F9W8