Detailed information of TA system TA129066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 2718573..2719181
• Replicon: chromosome
• Accession: NZ_CP041731
• Organism: Paenibacillus kribbensis strain PS04

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: FOA15_RS12265
• Protein ID: WP_007429335.1
• Coordinates: 2718831..2719181 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FOA15_RS12260
• Protein ID: WP_170970806.1
• Coordinates: 2718573..2718824 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA15_RS12245	2714712..2715344	+	633	WP_179031842.1	LysE family transporter	-
FOA15_RS12250	2715493..2716767	+	1275	WP_179031843.1	outer membrane lipoprotein carrier protein LolA	-
FOA15_RS12255	2717053..2718249	+	1197	WP_179031844.1	alanine racemase	-
FOA15_RS12260	2718573..2718824	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
FOA15_RS12265	2718831..2719181	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOA15_RS12270	2719361..2721583	+	2223	WP_179031845.1	RNA-binding transcriptional accessory protein	-
FOA15_RS12275	2721654..2721788	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
FOA15_RS12280	2721932..2722420	+	489	WP_094155923.1	SprT family protein	-
FOA15_RS12295	2722815..2723696	-	882	WP_179031846.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T129066 WP_007429335.1 NZ_CP041731:2718831-2719181 [Paenibacillus kribbensis]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T129066 NZ_CP041731:2718831-2719181 [Paenibacillus kribbensis]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCAGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTCAGTCCTACCGTGATCGTGGCAGCCATCACCGCCCAGATTCAGAAGG
CAAAGCTGCCCACGCATGTGGAGATCGACGCGGCAACGCATGGCTTTGATCGCGATTCTGTCGTTCTTCTGGAGCAAATT
CGGACGATCGACAAACAAAGGCTCACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
TCAAATCAGTCTTGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT129066 WP_170970806.1 NZ_CP041731:2718573-2718824 [Paenibacillus kribbensis]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT129066 NZ_CP041731:2718573-2718824 [Paenibacillus kribbensis]
ATGATCAGTTTGCCGGATCATCTCTTGCAGGAAGTGGATGGGATCGTAGCTATGGAAAATTCCAACCGTAGTGAATTTAT
TAGGCAGGCCATGAAGCTGTATGTAGGCGAACGTAAAAAACGTTACATTCGTGAAGCGATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACTTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAAGATGCTAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1