Detailed information of TA system TA129028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 24165..24754
• Replicon: plasmid unnamed2
• Accession: NZ_CP041718
• Organism: Acidiphilium multivorum strain JZ-6

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A5FUB2
• Locus tag: FE249_RS19455
• Protein ID: WP_011930859.1
• Coordinates: 24428..24754 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: FE249_RS19450
• Protein ID: WP_225487737.1
• Coordinates: 24165..24428 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE249_RS19420 (FE249_19305)	19970..20278	+	309	WP_142525672.1	mobilization protein	-
FE249_RS19425 (FE249_19310)	20365..20790	-	426	WP_142525671.1	type II toxin-antitoxin system VapC family toxin	-
FE249_RS19430 (FE249_19315)	20790..21044	-	255	WP_142525670.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FE249_RS19435 (FE249_19320)	21758..22999	-	1242	WP_225487734.1	replication initiator protein A	-
FE249_RS19440 (FE249_19325)	22986..23261	-	276	WP_211477237.1	CopG family transcriptional regulator	-
FE249_RS19445 (FE249_19330)	23276..23935	-	660	WP_225487735.1	AAA family ATPase	-
FE249_RS19450 (FE249_19335)	24165..24428	+	264	WP_225487737.1	antitoxin	Antitoxin
FE249_RS19455 (FE249_19340)	24428..24754	+	327	WP_011930859.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FE249_RS19460	24980..25327	-	348	WP_225487739.1	hypothetical protein	-
FE249_RS19465	25414..25575	-	162	WP_225487741.1	hypothetical protein	-
FE249_RS19470 (FE249_19345)	26031..26717	+	687	WP_142524345.1	PEP-CTERM sorting domain-containing protein	-
FE249_RS19475 (FE249_19350)	27233..27835	+	603	WP_225487743.1	PEP-CTERM sorting domain-containing protein	-
FE249_RS19480 (FE249_19355)	27916..28497	-	582	WP_225487744.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34418	34418
	-	flank	IS/Tn	-	-	27916..28497	581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11634.36 Da
• Isoelectric Point: 7.1341

>T129028 WP_011930859.1 NZ_CP041718:24428-24754 [Acidiphilium multivorum]
MNRGDIYLVDLEPTAGHEQRGHRPVLVVSPDAFNRLTQCPVILPITNGGDFARRVGFAVPISGIKTTGVVRCDQPRALDL
SARNARKVDTLPAAILDDVLATAATLFE

Nucleotide

• Length: 327 bp

>T129028 NZ_CP041718:24428-24754 [Acidiphilium multivorum]
ATGAATCGCGGCGATATCTATCTGGTCGATCTTGAGCCGACAGCAGGACACGAGCAGCGCGGCCATCGGCCGGTGCTGGT
CGTGTCCCCGGATGCCTTCAACCGCCTGACACAATGCCCGGTCATCCTGCCGATAACCAACGGCGGCGATTTCGCCAGGC
GTGTCGGTTTCGCGGTTCCAATCAGCGGCATCAAAACAACAGGCGTCGTGCGGTGCGATCAACCGCGCGCGCTCGATCTG
AGCGCCCGCAATGCTCGCAAGGTGGACACGCTGCCGGCGGCGATCCTGGATGACGTACTAGCCACGGCAGCCACGCTGTT
CGAGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9319.86 Da
• Isoelectric Point: 5.0210

>AT129028 WP_225487737.1 NZ_CP041718:24165-24428 [Acidiphilium multivorum]
MHTTNLRKVGGSIMLAVPPALLDVLHLAPGTRVGLAVDNGRLVVEPQARPRYTMADLLAEAEAAGVYPLPPEEREWIDAP
AVGKELI

Nucleotide

• Length: 264 bp

>AT129028 NZ_CP041718:24165-24428 [Acidiphilium multivorum]
ATGCACACCACAAACTTGCGCAAGGTCGGCGGGTCTATCATGCTAGCCGTGCCACCCGCACTGCTCGATGTCCTGCACCT
GGCACCGGGTACCAGGGTCGGCCTGGCTGTCGATAACGGCCGCCTGGTCGTCGAGCCGCAAGCCCGGCCGCGCTACACGA
TGGCCGATCTGCTCGCCGAGGCCGAAGCAGCCGGCGTCTATCCGCTGCCGCCGGAAGAACGCGAATGGATCGACGCCCCG
GCAGTTGGAAAAGAACTGATTTGA

Structures

Toxin

• AlphaFold DB: A5FUB2