Detailed information of TA system TA129022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 4550800..4551503
• Replicon: chromosome
• Accession: NZ_CP041695
• Organism: Nocardia otitidiscaviarum strain NEB252

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOH10_RS20325
• Protein ID: WP_143981933.1
• Coordinates: 4550800..4551222 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOH10_RS20330
• Protein ID: WP_143981934.1
• Coordinates: 4551219..4551503 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH10_RS20300	4545968..4547212	+	1245	WP_143984219.1	aldolase	-
FOH10_RS20305	4547209..4548240	+	1032	WP_143981931.1	allantoicase	-
FOH10_RS20310	4548270..4549154	+	885	WP_029928138.1	allantoinase PuuE	-
FOH10_RS20315	4549299..4549781	-	483	WP_143981932.1	hypothetical protein	-
FOH10_RS20320	4549918..4550658	-	741	WP_186000916.1	4-hydroxy-tetrahydrodipicolinate reductase	-
FOH10_RS20325	4550800..4551222	-	423	WP_143981933.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOH10_RS20330	4551219..4551503	-	285	WP_143981934.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FOH10_RS20335	4551589..4552704	-	1116	WP_143981935.1	alcohol dehydrogenase catalytic domain-containing protein	-
FOH10_RS20340	4552785..4553438	+	654	WP_143981936.1	class I SAM-dependent methyltransferase	-
FOH10_RS20345	4553443..4554942	-	1500	WP_143984221.1	peptidoglycan DD-metalloendopeptidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (13-76)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15758.92 Da
• Isoelectric Point: 4.8161

>T129022 WP_143981933.1 NZ_CP041695:c4551222-4550800 [Nocardia otitidiscaviarum]
VNFLLDTNAVSEWVKPQPNRGLADWFDSIDEDRTFLSVVTLGELRRGVDRLPDGKRRERLSAWLTEEVPDRFAGRLLPID
EVVALAWGRLRARVDVLGRCVDPVDGLIAATAESHGLTIVTRNVRDFEVTGVPIVDPWTR

Nucleotide

• Length: 423 bp

>T129022 NZ_CP041695:c4551222-4550800 [Nocardia otitidiscaviarum]
GTGAATTTTCTCCTCGACACCAACGCGGTATCGGAATGGGTGAAGCCCCAACCGAATCGAGGGCTGGCCGACTGGTTCGA
CTCGATCGACGAGGACCGCACATTCCTCAGCGTCGTGACGCTCGGTGAATTGCGTAGGGGCGTCGACAGACTGCCGGACG
GCAAACGGCGGGAGCGGCTGTCCGCATGGTTGACGGAGGAGGTGCCGGATCGTTTCGCGGGTCGGCTGCTACCGATCGAC
GAGGTCGTCGCGCTGGCGTGGGGACGATTGCGGGCCCGGGTGGACGTGCTGGGCCGCTGCGTCGACCCGGTCGACGGACT
CATCGCCGCGACCGCCGAATCTCACGGGCTCACGATTGTGACCCGCAATGTGCGGGACTTCGAAGTGACCGGCGTGCCGA
TCGTCGATCCGTGGACCCGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10793.25 Da
• Isoelectric Point: 10.2714

>AT129022 WP_143981934.1 NZ_CP041695:c4551503-4551219 [Nocardia otitidiscaviarum]
MVDEARRTRMDVAWPLAEAKAKLSELIDKVEREGPQVISRRGREVAVVVPVDEWRRKSARTGSLAQFFAESPLRDSGVVI
ERERSDSHHRDVRL

Nucleotide

• Length: 285 bp

>AT129022 NZ_CP041695:c4551503-4551219 [Nocardia otitidiscaviarum]
ATGGTGGACGAGGCTCGACGAACCCGCATGGACGTCGCGTGGCCGCTGGCGGAGGCCAAAGCCAAGCTGTCCGAATTGAT
CGACAAGGTCGAACGGGAGGGACCGCAGGTGATCAGCAGGCGCGGACGTGAGGTGGCCGTCGTGGTGCCGGTGGACGAGT
GGCGTCGGAAAAGTGCGCGCACCGGTAGTCTGGCTCAGTTCTTCGCCGAATCGCCGCTACGTGACTCCGGGGTGGTGATC
GAGCGCGAGCGCAGTGATTCCCACCACCGGGACGTCCGGCTGTGA