Detailed information of TA system TA129012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 372168..372805
• Replicon: chromosome
• Accession: NZ_CP041693
• Organism: Bacillus amyloliquefaciens strain H

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FOG69_RS01880
• Protein ID: WP_003156187.1
• Coordinates: 372455..372805 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: FOG69_RS01875
• Protein ID: WP_003156188.1
• Coordinates: 372168..372449 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOG69_RS01855	368534..369133	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
FOG69_RS01860	369226..369591	+	366	WP_013351093.1	holo-ACP synthase	-
FOG69_RS01865	369756..370763	+	1008	WP_013351094.1	outer membrane lipoprotein carrier protein LolA	-
FOG69_RS01870	370880..372049	+	1170	WP_013351095.1	alanine racemase	-
FOG69_RS01875	372168..372449	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FOG69_RS01880	372455..372805	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FOG69_RS01885	372926..373747	+	822	WP_013351096.1	STAS domain-containing protein	-
FOG69_RS01890	373752..374117	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
FOG69_RS01895	374120..374521	+	402	WP_013351097.1	anti-sigma regulatory factor	-
FOG69_RS01900	374533..375540	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
FOG69_RS01905	375604..375933	+	330	WP_014469786.1	anti-sigma factor antagonist	-
FOG69_RS01910	375930..376412	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
FOG69_RS01915	376378..377166	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
FOG69_RS01920	377166..377768	+	603	WP_014469787.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T129012 WP_003156187.1 NZ_CP041693:372455-372805 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T129012 NZ_CP041693:372455-372805 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT129012 WP_003156188.1 NZ_CP041693:372168-372449 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT129012 NZ_CP041693:372168-372449 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5