Detailed information of TA system TA128994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1295322..1296239
• Replicon: chromosome
• Accession: NZ_CP041691
• Organism: Bacillus amyloliquefaciens strain ZJU1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: FGF55_RS06875
• Protein ID: WP_007407256.1
• Coordinates: 1295493..1296239 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: FGF55_RS06870
• Protein ID: WP_014417527.1
• Coordinates: 1295322..1295492 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGF55_RS06825	1290528..1291040	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
FGF55_RS06830	1291153..1291812	+	660	WP_014417523.1	terminase	-
FGF55_RS06835	1291710..1292177	+	468	Protein_1274	hypothetical protein	-
FGF55_RS06840	1292190..1292561	+	372	WP_014417525.1	hypothetical protein	-
FGF55_RS06845	1292566..1292763	+	198	WP_007610833.1	XkdX family protein	-
FGF55_RS06850	1292820..1293581	+	762	WP_014417526.1	hypothetical protein	-
FGF55_RS06855	1293633..1293896	+	264	WP_003154815.1	hemolysin XhlA family protein	-
FGF55_RS06860	1293910..1294173	+	264	WP_003154813.1	phage holin	-
FGF55_RS06865	1294187..1295065	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
FGF55_RS06870	1295322..1295492	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
FGF55_RS06875	1295493..1296239	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FGF55_RS06880	1296343..1297341	-	999	WP_003154805.1	inorganic phosphate transporter	-
FGF55_RS06885	1297354..1297971	-	618	WP_003154804.1	DUF47 domain-containing protein	-
FGF55_RS06890	1298257..1299573	-	1317	WP_007610842.1	amino acid permease	-
FGF55_RS06895	1299895..1300845	+	951	WP_014417529.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T128994 WP_007407256.1 NZ_CP041691:c1296239-1295493 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T128994 NZ_CP041691:c1296239-1295493 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT128994 WP_014417527.1 NZ_CP041691:c1295492-1295322 [Bacillus amyloliquefaciens]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT128994 NZ_CP041691:c1295492-1295322 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8