Detailed information of TA system TA128993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 479071..479708
• Replicon: chromosome
• Accession: NZ_CP041691
• Organism: Bacillus amyloliquefaciens strain ZJU1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: FGF55_RS02430
• Protein ID: WP_003156187.1
• Coordinates: 479358..479708 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: FGF55_RS02425
• Protein ID: WP_003156188.1
• Coordinates: 479071..479352 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGF55_RS02405	475436..476035	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
FGF55_RS02410	476128..476493	+	366	WP_014304402.1	holo-ACP synthase	-
FGF55_RS02415	476658..477665	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
FGF55_RS02420	477782..478951	+	1170	WP_014416966.1	alanine racemase	-
FGF55_RS02425	479071..479352	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
FGF55_RS02430	479358..479708	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
FGF55_RS02435	479826..480647	+	822	WP_003156182.1	STAS domain-containing protein	-
FGF55_RS02440	480652..481017	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
FGF55_RS02445	481020..481421	+	402	WP_003156178.1	anti-sigma regulatory factor	-
FGF55_RS02450	481433..482440	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
FGF55_RS02455	482504..482833	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
FGF55_RS02460	482830..483312	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
FGF55_RS02465	483278..484066	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
FGF55_RS02470	484066..484668	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T128993 WP_003156187.1 NZ_CP041691:479358-479708 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T128993 NZ_CP041691:479358-479708 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT128993 WP_003156188.1 NZ_CP041691:479071-479352 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT128993 NZ_CP041691:479071-479352 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5