Detailed information of TA system TA128983

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4462721..4463556
• Replicon: chromosome
• Accession: NZ_CP041678
• Organism: Escherichia coli strain ESBL 15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: FOA56_RS22215
• Protein ID: WP_000854821.1
• Coordinates: 4463179..4463556 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FOA56_RS22210
• Protein ID: WP_032216526.1
• Coordinates: 4462721..4463089 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA56_RS22165	4457844..4458728	+	885	WP_032216515.1	50S ribosome-binding GTPase	-
FOA56_RS22170	4458847..4459524	+	678	WP_032223228.1	hypothetical protein	-
FOA56_RS22175	4459530..4459763	+	234	WP_032289278.1	DUF905 family protein	-
FOA56_RS22185	4459862..4460680	+	819	WP_053902307.1	DUF945 domain-containing protein	-
FOA56_RS22190	4460772..4461257	+	486	WP_032216512.1	antirestriction protein	-
FOA56_RS22195	4461273..4461749	+	477	WP_001186724.1	RadC family protein	-
FOA56_RS22200	4461818..4462039	+	222	WP_001601167.1	DUF987 domain-containing protein	-
FOA56_RS22205	4462058..4462702	+	645	Protein_4253	antitoxin of toxin-antitoxin stability system	-
FOA56_RS22210	4462721..4463089	+	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FOA56_RS22215	4463179..4463556	+	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
FOA56_RS22220	4463553..4463702	+	150	Protein_4256	hypothetical protein	-
FOA56_RS22225	4463778..4463975	+	198	WP_114141299.1	DUF957 domain-containing protein	-
FOA56_RS22230	4464060..4464902	+	843	WP_032216511.1	DUF4942 domain-containing protein	-
FOA56_RS22235	4465651..4467189	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T128983 WP_000854821.1 NZ_CP041678:4463179-4463556 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T128983 NZ_CP041678:4463179-4463556 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT128983 WP_032216526.1 NZ_CP041678:4462721-4463089 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT128983 NZ_CP041678:4462721-4463089 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCTCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGTCGACCGCGCCGGCATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54