Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3677536..3678154 Replicon chromosome
Accession NZ_CP041678
Organism Escherichia coli strain ESBL 15

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FOA56_RS18485 Protein ID WP_001291435.1
Coordinates 3677936..3678154 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FOA56_RS18480 Protein ID WP_000344800.1
Coordinates 3677536..3677910 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOA56_RS18470 3672626..3673819 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FOA56_RS18475 3673842..3676991 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FOA56_RS18480 3677536..3677910 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FOA56_RS18485 3677936..3678154 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FOA56_RS18490 3678325..3678876 + 552 WP_000102564.1 maltose O-acetyltransferase -
FOA56_RS18495 3678992..3679462 + 471 WP_000136192.1 YlaC family protein -
FOA56_RS18500 3679626..3681176 + 1551 WP_053902297.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FOA56_RS18505 3681218..3681571 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FOA56_RS18515 3681950..3682261 + 312 WP_000409911.1 MGMT family protein -
FOA56_RS18520 3682292..3682864 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T128978 WP_001291435.1 NZ_CP041678:3677936-3678154 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T128978 NZ_CP041678:3677936-3678154 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT128978 WP_000344800.1 NZ_CP041678:3677536-3677910 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT128978 NZ_CP041678:3677536-3677910 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References