Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1329701..1330326 Replicon chromosome
Accession NZ_CP041678
Organism Escherichia coli strain ESBL 15

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag FOA56_RS06505 Protein ID WP_000911329.1
Coordinates 1329928..1330326 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FOA56_RS06500 Protein ID WP_000450524.1
Coordinates 1329701..1329928 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOA56_RS06475 1325503..1325973 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FOA56_RS06480 1325973..1326545 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FOA56_RS06485 1326691..1327569 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FOA56_RS06490 1327586..1328620 + 1035 WP_001440783.1 outer membrane protein assembly factor BamC -
FOA56_RS06495 1328833..1329546 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FOA56_RS06500 1329701..1329928 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FOA56_RS06505 1329928..1330326 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
FOA56_RS06510 1330473..1331336 + 864 WP_001267498.1 neutral zinc metallopeptidase -
FOA56_RS06515 1331351..1333366 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
FOA56_RS06520 1333440..1334138 + 699 WP_000679823.1 esterase -
FOA56_RS06525 1334248..1334448 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T128967 WP_000911329.1 NZ_CP041678:1329928-1330326 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T128967 NZ_CP041678:1329928-1330326 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT128967 WP_000450524.1 NZ_CP041678:1329701-1329928 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT128967 NZ_CP041678:1329701-1329928 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References