Detailed information of TA system TA128962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 45041..45626
• Replicon: plasmid unnamed
• Accession: NZ_CP041677
• Organism: Limosilactobacillus reuteri strain LL7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FOD75_RS10870
• Protein ID: WP_144227885.1
• Coordinates: 45041..45397 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FOD75_RS10875
• Protein ID: WP_144227888.1
• Coordinates: 45390..45626 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOD75_RS10860	40579..43182	+	2604	WP_144227879.1	YfhO family protein	-
FOD75_RS10865	43204..44877	+	1674	WP_144227882.1	N-6 DNA methylase	-
FOD75_RS10870	45041..45397	-	357	WP_144227885.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOD75_RS10875	45390..45626	-	237	WP_144227888.1	AbrB family transcriptional regulator	Antitoxin
FOD75_RS10880	46101..46361	+	261	WP_144227891.1	type II toxin-antitoxin system PrlF family antitoxin	-
FOD75_RS10885	46361..46705	+	345	WP_144227894.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FOD75_RS10890	47318..47587	+	270	WP_144227897.1	CPBP family intramembrane metalloprotease	-
FOD75_RS10895	47702..48619	-	918	WP_144227900.1	M48 family metallopeptidase	-
FOD75_RS10900	49113..49352	+	240	WP_144227903.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..178977	178977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-102)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13584.64 Da
• Isoelectric Point: 9.7489

>T128962 WP_144227885.1 NZ_CP041677:c45397-45041 [Limosilactobacillus reuteri]
MIKQADIVTVDFEPSKGHEIQKRRPALVMSRNEYNKSTPLIIVCPITSTQRNRPFLIPLKQPVKDGTLDKQSKVNTIQVY
SLDSTKQGGRNVQVIGHLDDDEFYVIAQYLMQNFNFKF

Nucleotide

• Length: 357 bp

>T128962 NZ_CP041677:c45397-45041 [Limosilactobacillus reuteri]
ATGATTAAACAAGCTGATATTGTTACGGTTGATTTTGAACCTTCAAAAGGGCACGAAATTCAAAAAAGGCGTCCCGCACT
CGTGATGAGCCGCAATGAATATAATAAGAGCACTCCCCTAATCATTGTTTGCCCAATTACATCCACCCAAAGGAATCGAC
CATTTCTGATTCCGCTAAAGCAACCTGTAAAAGATGGCACATTAGATAAACAAAGTAAGGTTAACACAATTCAAGTCTAT
TCTCTTGATAGTACCAAACAAGGCGGGAGAAACGTTCAGGTTATCGGTCATCTTGATGACGATGAATTCTATGTAATTGC
ACAATACCTGATGCAAAATTTTAATTTCAAATTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8612.58 Da
• Isoelectric Point: 4.3569

>AT128962 WP_144227888.1 NZ_CP041677:c45626-45390 [Limosilactobacillus reuteri]
MDTVKTRQQGNTIAVTLSKKFNIPAGEEYYITKSDTGVITLVPKVKDIFVNVQEGQYSDADEDGLARNFTPKGSELDD

Nucleotide

• Length: 237 bp

>AT128962 NZ_CP041677:c45626-45390 [Limosilactobacillus reuteri]
ATGGACACCGTAAAAACCAGACAACAAGGCAATACGATTGCTGTCACACTTTCCAAGAAGTTCAATATACCAGCCGGCGA
AGAATACTACATTACGAAGTCCGATACGGGGGTAATTACCTTGGTTCCCAAGGTGAAAGATATTTTCGTTAATGTTCAAG
AAGGTCAATATAGCGACGCTGATGAAGATGGTCTTGCTCGCAACTTTACACCAAAAGGATCTGAATTAGATGATTAA