Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 444024..444660 Replicon chromosome
Accession NZ_CP041666
Organism Radiobacillus deserti strain TKL69

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag FN924_RS02355 Protein ID WP_143891902.1
Coordinates 444310..444660 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag FN924_RS02350 Protein ID WP_143891901.1
Coordinates 444024..444305 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FN924_RS02330 439536..439892 + 357 WP_143891897.1 holo-ACP synthase -
FN924_RS02335 439980..441497 + 1518 WP_143891898.1 NAD(P)H-hydrate dehydratase -
FN924_RS02340 441546..442565 + 1020 WP_143891899.1 outer membrane lipoprotein carrier protein LolA -
FN924_RS02345 442721..443839 + 1119 WP_143891900.1 alanine racemase -
FN924_RS02350 444024..444305 + 282 WP_143891901.1 antitoxin Antitoxin
FN924_RS02355 444310..444660 + 351 WP_143891902.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FN924_RS02360 444873..446096 + 1224 WP_143897093.1 ISL3 family transposase -
FN924_RS02365 446343..447212 + 870 WP_143891903.1 RsbT co-antagonist protein RsbRA -
FN924_RS02370 447217..447573 + 357 WP_143891904.1 STAS domain-containing protein -
FN924_RS02375 447582..447983 + 402 WP_143897094.1 anti-sigma regulatory factor -
FN924_RS02380 448030..449022 + 993 WP_143891905.1 PP2C family protein-serine/threonine phosphatase -
FN924_RS02385 449088..449417 + 330 WP_143891906.1 STAS domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 444891..446096 1205


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13017.05 Da        Isoelectric Point: 5.1409

>T128961 WP_143891902.1 NZ_CP041666:444310-444660 [Radiobacillus deserti]
MIVKRGEVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAQKYGFERDSVILLEQI
RTIDKQRLTDKITQLDQYMMENINKALEISLGLTDF

Download         Length: 351 bp

>T128961 NZ_CP041666:444310-444660 [Radiobacillus deserti]
TTGATTGTTAAAAGAGGCGAAGTCTATTTCGCCGATCTCTCTCCTGTTGTTGGTTCCGAGCAGGGGGGCGTCCGTCCAGT
ACTGGTGCTGCAAAACGATATAGGTAATCGTTTTAGTCCAACGGTTATTGTTGCAGCCATTACCGCGCAGATACAAAAAG
CGAAGTTACCTACTCATGTTGAAATTGACGCGCAGAAGTATGGATTTGAACGGGATTCTGTTATTCTTTTAGAACAAATT
AGAACCATTGATAAACAAAGACTAACTGATAAGATAACACAACTTGACCAATATATGATGGAAAATATTAACAAGGCTCT
CGAAATTAGCCTTGGATTAACTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10676.18 Da        Isoelectric Point: 4.7133

>AT128961 WP_143891901.1 NZ_CP041666:444024-444305 [Radiobacillus deserti]
MVLSESLQEIVVRLPKNLLNEVDGLMRSENSDLSDFICQATKSYLREKKKRHIRESMQQGYMEMAKINLNIASEAFQAEE
EAENTLERLVSGV

Download         Length: 282 bp

>AT128961 NZ_CP041666:444024-444305 [Radiobacillus deserti]
ATGGTTTTGTCAGAGAGCTTGCAAGAGATTGTAGTTAGACTACCAAAAAACCTACTAAATGAGGTGGACGGTTTAATGAG
AAGTGAGAATAGTGATCTCAGTGATTTCATTTGCCAGGCAACGAAATCGTATTTGCGTGAAAAGAAAAAACGCCATATTC
GGGAGTCCATGCAGCAAGGCTATATGGAGATGGCAAAAATAAATCTCAACATTGCTTCAGAAGCTTTTCAGGCTGAAGAG
GAGGCCGAAAACACCCTTGAGCGCTTAGTGAGCGGGGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References