Detailed information of TA system TA128944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 600956..601584
• Replicon: chromosome
• Accession: NZ_CP041657
• Organism: Lacticaseibacillus paracasei strain CBA3611

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: FGZ69_RS03365
• Protein ID: WP_012490993.1
• Coordinates: 600956..601324 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: FGZ69_RS03370
• Protein ID: WP_003583264.1
• Coordinates: 601324..601584 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGZ69_RS03345	596280..597287	-	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-
FGZ69_RS03350	597528..597797	+	270	WP_012490996.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
FGZ69_RS03355	598113..598931	+	819	WP_012490995.1	Cof-type HAD-IIB family hydrolase	-
FGZ69_RS03360	599295..600665	+	1371	WP_012490994.1	FAD-dependent oxidoreductase	-
FGZ69_RS03365	600956..601324	+	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGZ69_RS03370	601324..601584	+	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
FGZ69_RS03375	601697..602386	-	690	WP_012490992.1	ABC transporter ATP-binding protein	-
FGZ69_RS03380	602391..603452	-	1062	WP_003563374.1	ABC transporter permease	-
FGZ69_RS03385	603457..603960	-	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
FGZ69_RS03390	604211..604837	-	627	WP_003577713.1	flavodoxin	-
FGZ69_RS03395	604950..605693	-	744	WP_012490990.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T128944 WP_012490993.1 NZ_CP041657:600956-601324 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T128944 NZ_CP041657:600956-601324 [Lacticaseibacillus paracasei]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCAAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGTCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT128944 WP_003583264.1 NZ_CP041657:601324-601584 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT128944 NZ_CP041657:601324-601584 [Lacticaseibacillus paracasei]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A806L3G4