Detailed information of TA system TA128942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1161676..1162216
• Replicon: chromosome
• Accession: NZ_CP041656
• Organism: Bradyrhizobium cosmicum strain 58S1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FNV92_RS05750
• Protein ID: WP_015684160.1
• Coordinates: 1161956..1162216 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FNV92_RS05745
• Protein ID: WP_143841394.1
• Coordinates: 1161676..1161969 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNV92_RS05705	1156741..1157595	-	855	WP_143841392.1	3-mercaptopyruvate sulfurtransferase	-
FNV92_RS05710	1157728..1158141	-	414	WP_143841393.1	rhodanese-like domain-containing protein	-
FNV92_RS05715	1158270..1159445	-	1176	WP_015684164.1	VWA domain-containing protein	-
FNV92_RS05720	1159569..1159814	+	246	WP_015684163.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
FNV92_RS05725	1159908..1160750	+	843	WP_015684162.1	MoxR family ATPase	-
FNV92_RS05745	1161676..1161969	-	294	WP_143841394.1	BrnA antitoxin family protein	Antitoxin
FNV92_RS05750	1161956..1162216	-	261	WP_015684160.1	BrnT family toxin	Toxin
FNV92_RS05755	1162320..1163966	-	1647	WP_143841395.1	GMC family oxidoreductase N-terminal domain-containing protein	-
FNV92_RS05760	1163984..1164577	-	594	WP_015684158.1	GNAT family N-acetyltransferase	-
FNV92_RS05765	1164695..1165621	-	927	WP_015684157.1	SMP-30/gluconolactonase/LRE family protein	-
FNV92_RS05770	1165823..1166926	+	1104	WP_143841396.1	mandelate racemase/muconate lactonizing enzyme family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-79)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-92)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9672.20 Da
• Isoelectric Point: 10.3460

>T128942 WP_015684160.1 NZ_CP041656:c1162216-1161956 [Bradyrhizobium cosmicum]
MKIVWDEPKRLANLDKHGLDFADLDEAFFDTALVIPSQSNSKRWIAVGASIRGVIVVVFARLGREGVSIISMRPASRSER
KLYAER

Nucleotide

• Length: 261 bp

>T128942 NZ_CP041656:c1162216-1161956 [Bradyrhizobium cosmicum]
ATGAAAATCGTCTGGGATGAACCGAAGCGGCTCGCCAACCTCGACAAGCATGGGCTGGATTTTGCCGATCTCGACGAAGC
CTTCTTCGACACCGCGCTGGTCATTCCGTCACAAAGCAACAGCAAGCGCTGGATCGCGGTCGGCGCGAGTATCCGTGGCG
TCATCGTCGTCGTATTCGCCCGGCTGGGACGCGAAGGCGTCAGCATCATCAGCATGCGACCGGCAAGCCGCAGCGAAAGG
AAACTCTATGCCGAACGCTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10864.52 Da
• Isoelectric Point: 11.2845

>AT128942 WP_143841394.1 NZ_CP041656:c1161969-1161676 [Bradyrhizobium cosmicum]
MPNARKKSNYTKKDLRAVSDNPELTKADFAKARPFSEVFPDLSASIRKGRGPNKAPTKKLVSLRLSPEVVEHFKSTGPGW
QSRIDETLRKAVKRKAS

Nucleotide

• Length: 294 bp

>AT128942 NZ_CP041656:c1161969-1161676 [Bradyrhizobium cosmicum]
ATGCCGAACGCTAGGAAGAAGTCGAACTATACAAAGAAGGACCTGCGCGCGGTCAGCGATAATCCGGAGCTGACCAAGGC
CGATTTCGCCAAGGCGCGGCCCTTCTCTGAAGTCTTCCCTGATCTCAGTGCATCTATCCGCAAGGGCCGGGGGCCGAACA
AGGCGCCGACAAAGAAGCTCGTCTCCCTCCGCCTCAGCCCGGAAGTGGTCGAGCATTTCAAATCGACCGGACCGGGCTGG
CAATCGCGGATCGACGAGACCTTGCGCAAGGCGGTGAAGCGCAAGGCGTCGTGA