Detailed information of TA system TA128939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 46359..47095
• Replicon: plasmid pKleb8A7
• Accession: NZ_CP041645
• Organism: Klebsiella pneumoniae strain NKU_Kleb8A7

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: FOA47_RS26570
• Protein ID: WP_004187044.1
• Coordinates: 46613..47095 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: FOA47_RS26565
• Protein ID: WP_003026799.1
• Coordinates: 46359..46625 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA47_RS26540	41508..42488	+	981	Protein_45	IS5 family transposase	-
FOA47_RS26545	42546..42791	+	246	WP_032238678.1	hypothetical protein	-
FOA47_RS26550	42760..45345	+	2586	WP_004187040.1	EAL domain-containing protein	-
FOA47_RS26555	45504..46208	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOA47_RS26565	46359..46625	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
FOA47_RS26570	46613..47095	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
FOA47_RS26575	47306..48652	+	1347	WP_077251107.1	ISNCY family transposase	-
FOA47_RS26580	48812..49516	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
FOA47_RS27520	49750..49857	-	108	Protein_53	IS3 family transposase	-
FOA47_RS26585	49855..50025	-	171	Protein_54	transposase	-
FOA47_RS26590	50135..51071	+	937	Protein_55	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1 / blaTEM-1B	-	1..114444	114444
	-	inside	IScluster/Tn	-	-	41508..51092	9584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T128939 WP_004187044.1 NZ_CP041645:46613-47095 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T128939 NZ_CP041645:46613-47095 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT128939 WP_003026799.1 NZ_CP041645:46359-46625 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT128939 NZ_CP041645:46359-46625 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66