Detailed information of TA system TA128938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 32942..33464
• Replicon: plasmid pKleb8A7
• Accession: NZ_CP041645
• Organism: Klebsiella pneumoniae strain NKU_Kleb8A7

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: FOA47_RS26470
• Protein ID: WP_004187019.1
• Coordinates: 32942..33226 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: FOA47_RS26475
• Protein ID: WP_004187025.1
• Coordinates: 33216..33464 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA47_RS26445	28161..28625	-	465	WP_004187010.1	hypothetical protein	-
FOA47_RS26455	30168..30692	+	525	Protein_28	hypothetical protein	-
FOA47_RS27510	30709..31192	+	484	Protein_29	hypothetical protein	-
FOA47_RS27515	31190..31489	+	300	Protein_30	conjugal transfer protein TraH	-
FOA47_RS26465	31571..32926	+	1356	WP_004187017.1	ISNCY family transposase	-
FOA47_RS26470	32942..33226	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOA47_RS26475	33216..33464	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
FOA47_RS26480	33754..34178	-	425	Protein_34	IS1 family transposase	-
FOA47_RS26485	34366..34482	-	117	Protein_35	transposase domain-containing protein	-
FOA47_RS26490	34427..34690	-	264	WP_004187027.1	transposase	-
FOA47_RS26495	34705..34968	+	264	WP_004118208.1	hypothetical protein	-
FOA47_RS26500	35212..35493	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
FOA47_RS26505	35528..36097	+	570	WP_004152117.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catA1 / blaTEM-1B	-	1..114444	114444
	-	flank	IS/Tn	-	-	33718..33984	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T128938 WP_004187019.1 NZ_CP041645:c33226-32942 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T128938 NZ_CP041645:c33226-32942 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT128938 WP_004187025.1 NZ_CP041645:c33464-33216 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT128938 NZ_CP041645:c33464-33216 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4