Detailed information of TA system TA128919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 95538..96063
• Replicon: plasmid pKP27-NDM4
• Accession: NZ_CP041642
• Organism: Klebsiella pneumoniae strain PIMB15ND2KP27

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: DIJ67_RS29020
• Protein ID: WP_013023785.1
• Coordinates: 95758..96063 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: DIJ67_RS29015
• Protein ID: WP_001568025.1
• Coordinates: 95538..95756 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIJ67_RS28965	90928..91131	-	204	WP_004197809.1	hypothetical protein	-
DIJ67_RS28970	91145..91348	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
DIJ67_RS28975	91382..91750	-	369	WP_004197807.1	hypothetical protein	-
DIJ67_RS28980	91794..92288	-	495	WP_044816180.1	hypothetical protein	-
DIJ67_RS28985	92319..92891	-	573	WP_020805590.1	hypothetical protein	-
DIJ67_RS28990	92888..93136	-	249	WP_000272716.1	hypothetical protein	-
DIJ67_RS28995	93573..94262	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
DIJ67_RS29000	94294..94983	-	690	WP_004193995.1	hypothetical protein	-
DIJ67_RS29015	95538..95756	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
DIJ67_RS29020	95758..96063	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
DIJ67_RS29025	96232..96627	+	396	WP_017899885.1	hypothetical protein	-
DIJ67_RS29030	96654..96968	+	315	WP_053389906.1	hypothetical protein	-
DIJ67_RS29035	96979..97995	+	1017	WP_017899884.1	hypothetical protein	-
DIJ67_RS29040	98193..98987	+	795	WP_004197635.1	site-specific integrase	-
DIJ67_RS29045	99467..99646	-	180	WP_006788217.1	hypothetical protein	-
DIJ67_RS29050	99766..100392	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-4 / aac(3)-IId / blaCTX-M-14 / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaLAP-2 / qnrS1	htpB	1..121851	121851

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T128919 WP_013023785.1 NZ_CP041642:95758-96063 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T128919 NZ_CP041642:95758-96063 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT128919 WP_001568025.1 NZ_CP041642:95538-95756 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT128919 NZ_CP041642:95538-95756 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8