Detailed information of TA system TA128917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 108783..109528
• Replicon: plasmid pKP27-MPH
• Accession: NZ_CP041640
• Organism: Klebsiella pneumoniae strain PIMB15ND2KP27

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: DIJ67_RS26690
• Protein ID: WP_032408901.1
• Coordinates: 108783..109274 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: DIJ67_RS26695
• Protein ID: WP_014386183.1
• Coordinates: 109262..109528 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIJ67_RS26665	104467..105222	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
DIJ67_RS26670	105484..105924	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
DIJ67_RS26675	105921..106271	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
DIJ67_RS26680	106302..107894	+	1593	Protein_110	IS66 family transposase	-
DIJ67_RS26685	108091..108342	+	252	WP_032408900.1	aldo/keto reductase	-
DIJ67_RS26690	108783..109274	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
DIJ67_RS26695	109262..109528	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
DIJ67_RS26700	109725..109883	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
DIJ67_RS26705	110127..110612	-	486	WP_014386185.1	hypothetical protein	-
DIJ67_RS26710	110802..111074	+	273	WP_032408902.1	hypothetical protein	-
DIJ67_RS26715	111071..111421	+	351	WP_014386186.1	hypothetical protein	-
DIJ67_RS26730	112058..112384	+	327	WP_014386187.1	hypothetical protein	-
DIJ67_RS26735	112446..112658	+	213	WP_014386188.1	hypothetical protein	-
DIJ67_RS26740	112669..112893	+	225	WP_014386189.1	hypothetical protein	-
DIJ67_RS26745	112974..113293	+	320	Protein_121	type II toxin-antitoxin system RelE/ParE family toxin	-
DIJ67_RS26750	113283..113561	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
DIJ67_RS26755	113562..113975	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / dfrA12 / aadA2 / qacE / sul1 / tet(A)	-	1..222330	222330

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T128917 WP_032408901.1 NZ_CP041640:c109274-108783 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T128917 NZ_CP041640:c109274-108783 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT128917 WP_014386183.1 NZ_CP041640:c109528-109262 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT128917 NZ_CP041640:c109528-109262 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6