Detailed information of TA system TA128916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 84989..85632
• Replicon: plasmid pKP27-MPH
• Accession: NZ_CP041640
• Organism: Klebsiella pneumoniae strain PIMB15ND2KP27

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: DIJ67_RS26545
• Protein ID: WP_014386165.1
• Coordinates: 84989..85405 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: DIJ67_RS26550
• Protein ID: WP_032408893.1
• Coordinates: 85402..85632 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIJ67_RS26520	81609..82040	+	432	WP_001568057.1	conjugation system SOS inhibitor PsiB	-
DIJ67_RS26525	82037..82765	+	729	WP_146326645.1	plasmid SOS inhibition protein A	-
DIJ67_RS26530	82762..83088	+	327	WP_001568059.1	hypothetical protein	-
DIJ67_RS26535	83144..83518	+	375	WP_001568060.1	hypothetical protein	-
DIJ67_RS26540	83698..84666	-	969	WP_186725998.1	IS5 family transposase	-
DIJ67_RS26545	84989..85405	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
DIJ67_RS26550	85402..85632	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DIJ67_RS26560	85952..86386	+	435	WP_000103648.1	RidA family protein	-
DIJ67_RS26565	86391..87065	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
DIJ67_RS26570	87081..88238	+	1158	WP_032408895.1	acetylornithine deacetylase	-
DIJ67_RS26575	88409..89557	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
DIJ67_RS26580	89564..90559	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / dfrA12 / aadA2 / qacE / sul1 / tet(A)	-	1..222330	222330
	-	flank	IS/Tn	-	-	83698..84666	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T128916 WP_014386165.1 NZ_CP041640:c85405-84989 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T128916 NZ_CP041640:c85405-84989 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT128916 WP_032408893.1 NZ_CP041640:c85632-85402 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT128916 NZ_CP041640:c85632-85402 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA