Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 10369..11105 Replicon plasmid pKP27-MPH
Accession NZ_CP041640
Organism Klebsiella pneumoniae strain PIMB15ND2KP27

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag DIJ67_RS26105 Protein ID WP_003026803.1
Coordinates 10369..10851 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DIJ67_RS26110 Protein ID WP_003026799.1
Coordinates 10839..11105 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DIJ67_RS26090 6826..8172 - 1347 WP_020314316.1 ISNCY family transposase -
DIJ67_RS26095 8368..8766 - 399 WP_032422684.1 helix-turn-helix domain-containing protein -
DIJ67_RS26100 8815..10161 - 1347 WP_077255522.1 ISNCY family transposase -
DIJ67_RS26105 10369..10851 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
DIJ67_RS26110 10839..11105 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DIJ67_RS26115 11281..11535 - 255 WP_004152108.1 hypothetical protein -
DIJ67_RS26120 11611..11868 - 258 WP_004152107.1 hypothetical protein -
DIJ67_RS26125 11917..12120 - 204 WP_004152106.1 hemolysin expression modulator Hha -
DIJ67_RS26130 12154..12522 - 369 WP_146326642.1 hypothetical protein -
DIJ67_RS26135 12566..13060 - 495 WP_073578691.1 DNA-binding protein -
DIJ67_RS26140 13091..13666 - 576 WP_004152103.1 hypothetical protein -
DIJ67_RS26145 13654..13923 - 270 WP_004152102.1 hypothetical protein -
DIJ67_RS26150 14281..14631 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
DIJ67_RS26155 14681..15043 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / dfrA12 / aadA2 / qacE / sul1 / tet(A) - 1..222330 222330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T128915 WP_003026803.1 NZ_CP041640:c10851-10369 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T128915 NZ_CP041640:c10851-10369 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT128915 WP_003026799.1 NZ_CP041640:c11105-10839 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT128915 NZ_CP041640:c11105-10839 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References