Detailed information of TA system TA128914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5169735..5170360
• Replicon: chromosome
• Accession: NZ_CP041639
• Organism: Klebsiella pneumoniae strain PIMB15ND2KP27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DIJ67_RS25670
• Protein ID: WP_040182550.1
• Coordinates: 5169735..5170118 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: DIJ67_RS25675
• Protein ID: WP_004150355.1
• Coordinates: 5170118..5170360 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIJ67_RS25655	5167101..5168003	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
DIJ67_RS25660	5168000..5168635	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
DIJ67_RS25665	5168632..5169561	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
DIJ67_RS25670	5169735..5170118	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
DIJ67_RS25675	5170118..5170360	-	243	WP_004150355.1	ribbon-helix-helix domain-containing protein	Antitoxin
DIJ67_RS25680	5170565..5171482	+	918	WP_023302328.1	alpha/beta hydrolase	-
DIJ67_RS25685	5171496..5172437	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
DIJ67_RS25690	5172482..5172919	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DIJ67_RS25695	5172916..5173776	-	861	WP_004146232.1	virulence factor BrkB family protein	-
DIJ67_RS25700	5173770..5174369	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T128914 WP_040182550.1 NZ_CP041639:c5170118-5169735 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T128914 NZ_CP041639:c5170118-5169735 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGAAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT128914 WP_004150355.1 NZ_CP041639:c5170360-5170118 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT128914 NZ_CP041639:c5170360-5170118 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9