Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3239422..3240058 Replicon chromosome
Accession NZ_CP041632
Organism Geobacillus sp. E263

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag FNL97_RS16530 Protein ID WP_003253417.1
Coordinates 3239422..3239772 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A178U3Z0
Locus tag FNL97_RS16535 Protein ID WP_003253419.1
Coordinates 3239777..3240058 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL97_RS16515 3236260..3236727 - 468 WP_062678763.1 SprT family protein -
FNL97_RS16520 3236816..3236929 + 114 WP_012748966.1 cortex morphogenetic protein CmpA -
FNL97_RS16525 3236924..3239110 - 2187 WP_143416199.1 RNA-binding transcriptional accessory protein -
FNL97_RS16530 3239422..3239772 - 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FNL97_RS16535 3239777..3240058 - 282 WP_003253419.1 hypothetical protein Antitoxin
FNL97_RS16540 3240187..3241350 - 1164 WP_062678764.1 alanine racemase -
FNL97_RS16545 3241574..3242587 - 1014 WP_143416200.1 outer membrane lipoprotein carrier protein LolA -
FNL97_RS16550 3242669..3243028 - 360 WP_012748962.1 holo-ACP synthase -
FNL97_RS16555 3243088..3243705 + 618 WP_062678766.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T128896 WP_003253417.1 NZ_CP041632:c3239772-3239422 [Geobacillus sp. E263]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T128896 NZ_CP041632:c3239772-3239422 [Geobacillus sp. E263]
TTGATTGTTAAGCGTGGCGACGTTTATTTTGCGGACCTTTCCCCGGTAGTGGGCTCAGAGCAAGGAGGCGTTCGTCCGGT
ATTGGTCATTCAAAATGACATTGGGAATCGATTTAGTCCGACGGTGATTGTGGCGGCAATTACAGCACAAATTCAAAAAG
CGAAGCTGCCAACGCATGTCGAAATTGATGCAAAACGTTACGGGTTTGAGCGGGATTCTGTCATTCTGCTAGAGCAAATT
CGGACAATTGATAAACAGCGGCTAACAGACAAAATTACTCACTTGGACGATGAAATGATGGATAAGGTGGACGAAGCGCT
GCAAATTAGTTTAGGACTTATAGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10631.16 Da        Isoelectric Point: 7.0142

>AT128896 WP_003253419.1 NZ_CP041632:c3240058-3239777 [Geobacillus sp. E263]
VSESSATTEIVVRLPQALVTELDVLVKQENGNRNDLIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFLAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT128896 NZ_CP041632:c3240058-3239777 [Geobacillus sp. E263]
GTGTCGGAATCTAGCGCAACAACAGAAATCGTCGTTCGTTTGCCTCAAGCATTGGTAACCGAATTAGATGTGCTTGTAAA
ACAGGAAAACGGCAACCGCAATGACCTTATTTATCAAGCGACTAAAATGTATATTCGCGAGCGAAAAAAACGTCAAATCC
GTGAAGCGATGAGACGCGGTTATATGGAAATGGCCAAAATCAATTTATCCATTGCTTCAGAAGCGTTTCTTGCAGAATAC
GAGGCCGACCACACCGTTGAACGTTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A178U3Z0

References