Detailed information of TA system TA128895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 353599..354293
• Replicon: chromosome
• Accession: NZ_CP041632
• Organism: Geobacillus sp. E263

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6N3WM91
• Locus tag: FNL97_RS01720
• Protein ID: WP_033020639.1
• Coordinates: 353907..354293 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FNL97_RS01715
• Protein ID: WP_062679016.1
• Coordinates: 353599..353907 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNL97_RS01695	349125..351233	+	2109	WP_143414909.1	SH3 domain-containing protein	-
FNL97_RS01700	351523..352703	+	1181	Protein_332	IS701 family transposase	-
FNL97_RS01705	352763..352996	+	234	Protein_333	Abortive infection protein AbiEi	-
FNL97_RS01710	352996..353295	+	300	Protein_334	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FNL97_RS01715	353599..353907	+	309	WP_062679016.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FNL97_RS01720	353907..354293	+	387	WP_033020639.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FNL97_RS01725	354608..355216	+	609	WP_062678595.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
FNL97_RS01730	355405..355794	+	390	WP_172713563.1	IS3 family transposase	-
FNL97_RS01735	355970..356119	+	150	WP_143416270.1	SH3 domain-containing protein	-
FNL97_RS01740	356960..358822	+	1863	WP_172713564.1	leucine-rich repeat domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	319464..358822	39358
	-	flank	IS/Tn	-	-	351523..352332	809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-116)

Antitoxin

• Antitoxin PemI-like (24-100)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14289.30 Da
• Isoelectric Point: 4.6190

>T128895 WP_033020639.1 NZ_CP041632:353907-354293 [Geobacillus sp. E263]
MPADVPERGDFVVLNFNPQAGHEQAGRRNAIVLSPKEFNQATGFIAVCPITNQKKGYPFEVDLPKEGISLDGDGYPITGV
VLTDQIKSLDWKARNLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Nucleotide

• Length: 387 bp

>T128895 NZ_CP041632:353907-354293 [Geobacillus sp. E263]
ATGCCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCAATCCACAAGCTGGTCATGAACAAGCTGGGAG
AAGGAATGCTATTGTTTTATCGCCTAAAGAATTCAATCAAGCAACAGGATTTATAGCTGTTTGTCCGATTACCAATCAAA
AGAAGGGTTATCCTTTCGAAGTGGATTTACCAAAAGAAGGAATATCCCTTGATGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACTGATCAAATTAAGTCATTGGATTGGAAAGCTCGTAATCTAAAAATTTTAAAGAAGTATAATCCAGAAGATGC
TCAAATTGAAGAAATAGATAAAATCATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11522.17 Da
• Isoelectric Point: 7.2044

>AT128895 WP_062679016.1 NZ_CP041632:353599-353907 [Geobacillus sp. E263]
MNSKEHLESKINQSKGGDYMSTVVAQKWGNSLGIRIPKDVADKIGIKQGSEMELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Nucleotide

• Length: 309 bp

>AT128895 NZ_CP041632:353599-353907 [Geobacillus sp. E263]
ATGAACTCAAAGGAACATTTAGAAAGCAAAATAAACCAAAGTAAAGGAGGGGACTACATGTCTACAGTTGTTGCTCAAAA
ATGGGGAAACAGTTTGGGCATTCGTATCCCAAAAGATGTTGCTGATAAAATAGGAATCAAACAAGGTTCCGAAATGGAAT
TAAACGTGATTGGAAATGAAGGTATCATCACATTAAAGCCAAAAAGAACACGGAAGAAATACACATTGGAAGAACTAATA
TCACAGATTACACCTGAAAATCGACACGAGGAAATTGATTTTGGGATAGAAGGGCGTGAATTGATTTAA

Structures

Toxin

• AlphaFold DB: A0A6N3WM91