Detailed information of TA system TA128894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 3372..3894
• Replicon: plasmid pPE15-27K
• Accession: NZ_CP041631
• Organism: Escherichia coli strain PE15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FNZ59_RS24305
• Protein ID: WP_112933652.1
• Coordinates: 3372..3653 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: FNZ59_RS24310
• Protein ID: WP_000121743.1
• Coordinates: 3643..3894 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNZ59_RS24270	608..1312	+	705	WP_001067845.1	IS6-like element IS26 family transposase	-
FNZ59_RS24275	1346..1537	-	192	WP_160268134.1	hypothetical protein	-
FNZ59_RS24280	1550..1699	-	150	WP_000003880.1	hypothetical protein	-
FNZ59_RS24285	1986..2315	-	330	WP_000866648.1	hypothetical protein	-
FNZ59_RS24290	2408..2623	-	216	WP_001180116.1	hypothetical protein	-
FNZ59_RS24295	2613..2858	-	246	WP_000356546.1	hypothetical protein	-
FNZ59_RS24300	2903..3226	-	324	WP_001181903.1	hypothetical protein	-
FNZ59_RS24305	3372..3653	-	282	WP_112933652.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FNZ59_RS24310	3643..3894	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
FNZ59_RS24315	5150..5986	+	837	WP_001050931.1	replication initiation protein	-
FNZ59_RS24320	6026..6472	+	447	WP_001074386.1	hypothetical protein	-
FNZ59_RS24325	6540..6962	+	423	WP_031942371.1	DNA distortion polypeptide 3	-
FNZ59_RS24330	7022..7990	-	969	WP_166501310.1	IS5 family transposase	-
FNZ59_RS24335	7992..8285	-	294	WP_015060510.1	hypothetical protein	-
FNZ59_RS24340	8301..8531	-	231	WP_000051064.1	partitioning protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / qnrS1 / tet(M) / blaTEM-1B	-	1..27108	27108

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10977.77 Da
• Isoelectric Point: 10.4466

>T128894 WP_112933652.1 NZ_CP041631:c3653-3372 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAAQLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T128894 NZ_CP041631:c3653-3372 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCAGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT128894 WP_000121743.1 NZ_CP041631:c3894-3643 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT128894 NZ_CP041631:c3894-3643 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S5I301