Detailed information of TA system TA128890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4314024..4314861
• Replicon: chromosome
• Accession: NZ_CP041628
• Organism: Escherichia coli strain PE15

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: FNZ59_RS20820
• Protein ID: WP_000227784.1
• Coordinates: 4314024..4314566 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: FNZ59_RS20825
• Protein ID: WP_001297137.1
• Coordinates: 4314550..4314861 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNZ59_RS20800	4309563..4310474	-	912	WP_000705853.1	2-dehydropantoate 2-reductase	-
FNZ59_RS20805	4310642..4311133	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
FNZ59_RS20810	4311261..4312625	-	1365	WP_160268115.1	MFS transporter	-
FNZ59_RS20815	4313033..4313968	+	936	WP_053900775.1	sel1 repeat family protein	-
FNZ59_RS20820	4314024..4314566	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
FNZ59_RS20825	4314550..4314861	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
FNZ59_RS20830	4315046..4315936	-	891	WP_000971336.1	heme o synthase	-
FNZ59_RS20835	4315948..4316277	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
FNZ59_RS20840	4316277..4316891	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
FNZ59_RS20845	4316881..4318872	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
FNZ59_RS20850	4318894..4319841	-	948	WP_001239434.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T128890 WP_000227784.1 NZ_CP041628:c4314566-4314024 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T128890 NZ_CP041628:c4314566-4314024 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT128890 WP_001297137.1 NZ_CP041628:c4314861-4314550 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT128890 NZ_CP041628:c4314861-4314550 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGCGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3