Detailed information of TA system TA128889

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4177707..4178505
• Replicon: chromosome
• Accession: NZ_CP041628
• Organism: Escherichia coli strain PE15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A166U9J9
• Locus tag: FNZ59_RS20155
• Protein ID: WP_000854695.1
• Coordinates: 4178128..4178505 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2U2UUE3
• Locus tag: FNZ59_RS20150
• Protein ID: WP_016243360.1
• Coordinates: 4177707..4178081 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNZ59_RS20120	4173017..4175863	+	2847	WP_127790593.1	Ag43/Cah family autotransporter adhesin	-
FNZ59_RS20125	4175934..4176167	+	234	WP_001117568.1	DUF905 family protein	-
FNZ59_RS20130	4176258..4176590	+	333	Protein_3922	DUF932 domain-containing protein	-
FNZ59_RS20135	4176592..4176762	+	171	Protein_3923	antirestriction protein	-
FNZ59_RS20140	4176778..4177254	+	477	WP_001186726.1	RadC family protein	-
FNZ59_RS20145	4177323..4177544	+	222	WP_000692307.1	DUF987 domain-containing protein	-
FNZ59_RS20150	4177707..4178081	+	375	WP_016243360.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
FNZ59_RS20155	4178128..4178505	+	378	WP_000854695.1	TA system toxin CbtA family protein	Toxin
FNZ59_RS20160	4178502..4178990	+	489	WP_000761710.1	hypothetical protein	-
FNZ59_RS20165	4179002..4179199	+	198	WP_000839228.1	DUF957 domain-containing protein	-
FNZ59_RS20170	4179284..4180132	+	849	WP_001280536.1	DUF4942 domain-containing protein	-
FNZ59_RS20175	4180225..4181544	-	1320	WP_000144689.1	site-specific integrase	-
FNZ59_RS20180	4181882..4182220	-	339	Protein_3932	LysR family transcriptional regulator	-
FNZ59_RS20185	4182271..4183227	-	957	WP_077491620.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14062.03 Da
• Isoelectric Point: 7.7531

>T128889 WP_000854695.1 NZ_CP041628:4178128-4178505 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPGAKQ

Nucleotide

• Length: 378 bp

>T128889 NZ_CP041628:4178128-4178505 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGCACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13810.66 Da
• Isoelectric Point: 7.0266

>AT128889 WP_016243360.1 NZ_CP041628:4177707-4178081 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT128889 NZ_CP041628:4177707-4178081 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGAGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A166U9J9

Antitoxin

• AlphaFold DB: A0A2U2UUE3