Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2172420..2173074 Replicon chromosome
Accession NZ_CP041628
Organism Escherichia coli strain PE15

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FNZ59_RS10655 Protein ID WP_000244777.1
Coordinates 2172420..2172827 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FNZ59_RS10660 Protein ID WP_000354046.1
Coordinates 2172808..2173074 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNZ59_RS10635 2168377..2170110 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
FNZ59_RS10640 2170116..2170826 - 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FNZ59_RS10645 2170851..2171747 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FNZ59_RS10650 2171859..2172380 + 522 WP_001055874.1 flavodoxin FldB -
FNZ59_RS10655 2172420..2172827 - 408 WP_000244777.1 toxin CptA Toxin
FNZ59_RS10660 2172808..2173074 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FNZ59_RS10665 2173317..2174297 + 981 WP_001625883.1 tRNA-modifying protein YgfZ -
FNZ59_RS10670 2174493..2175152 - 660 WP_000250274.1 hemolysin III family protein -
FNZ59_RS10675 2175316..2175627 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FNZ59_RS10680 2175672..2177105 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
FNZ59_RS10685 2177162..2177905 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T128880 WP_000244777.1 NZ_CP041628:c2172827-2172420 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T128880 NZ_CP041628:c2172827-2172420 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT128880 WP_000354046.1 NZ_CP041628:c2173074-2172808 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT128880 NZ_CP041628:c2173074-2172808 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTCAACTGGCTAATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References