Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 21406..22050 Replicon plasmid p35K_like
Accession NZ_CP041625
Organism Escherichia coli O157:H7 strain ATCC 43888

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag FNZ21_RS27705 Protein ID WP_000833471.1
Coordinates 21406..21588 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag FNZ21_RS27710 Protein ID WP_000466317.1
Coordinates 21613..22050 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNZ21_RS27665 18414..18737 + 324 WP_000432877.1 hypothetical protein -
FNZ21_RS27670 18778..19002 + 225 WP_000866040.1 hypothetical protein -
FNZ21_RS27675 19098..19322 + 225 WP_000749827.1 hypothetical protein -
FNZ21_RS27680 19373..19639 + 267 WP_000868275.1 hypothetical protein -
FNZ21_RS27685 19629..19871 + 243 WP_001450803.1 hypothetical protein -
FNZ21_RS27695 20311..20640 + 330 WP_000449469.1 hypothetical protein -
FNZ21_RS27700 20698..20976 + 279 WP_000545934.1 hypothetical protein -
FNZ21_RS27705 21406..21588 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
FNZ21_RS27710 21613..22050 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FNZ21_RS27715 22079..22783 - 705 WP_001279868.1 hypothetical protein -
FNZ21_RS27720 22780..23541 - 762 WP_001097937.1 conjugal transfer protein TraL -
FNZ21_RS27725 23551..23958 - 408 WP_001190619.1 hypothetical protein -
FNZ21_RS27730 24304..24639 + 336 WP_000963830.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32726 32726


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T128839 WP_000833471.1 NZ_CP041625:21406-21588 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T128839 NZ_CP041625:21406-21588 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT128839 WP_000466317.1 NZ_CP041625:21613-22050 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT128839 NZ_CP041625:21613-22050 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References